Evidence map›Paper›PMID 33543040›Full record

ArticleMatrix biology plus2020

Use of osteoblast-derived matrix to assess the influence of collagen modifications on cancer cells.

Neus Bota-Rabassedas, Hou-Fu Guo, Priyam Banerjee, Yulong Chen, Masahiko Terajima, Mitsuo Yamauchi, Jonathan M Kurie

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Matrix biology plus, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Fabrication ofPolymers · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Neus Bota-RabassedasDepartment of Thoracic/Head and Neck Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Hou-Fu GuoDepartment of Thoracic/Head and Neck Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Priyam BanerjeeDepartment of Thoracic/Head and Neck Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Yulong ChenGenitourinary Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Masahiko TerajimaOral and Craniofacial Health Sciences, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Mitsuo YamauchiOral and Craniofacial Health Sciences, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Jonathan M KurieDepartment of Thoracic/Head and Neck Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA.
The University of Texas MD Anderson Cancer Center · USUniversity of North Carolina at Chapel Hill · US

Funding

Regulation of Lung Cancer Metastasis by a Procollagen Lysine HydroxylaseR01CA105155 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI KURIE, JONATHAN M · 2004 to 2019
$4.0M
Regulation of lung cancer metastasis through transcriptional control of the Golgi apparatusR01CA211125 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI KURIE, JONATHAN M · 2017 to 2021
$1.8M
Regulation of Lung Cancer Metastasis by ZEB1R01CA181184 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI KURIE, JONATHAN M · 2014 to 2018
$1.7M
Determining structural features of a collagen lysyl hydroxylase that promotes lung cancer metastasisR00CA225633 · NCI · UNIVERSITY OF KENTUCKY · PI GUO, HOUFU · 2020 to 2022
$730k
Determining structural features of a collagen lysyl hydroxylase that promotes lung cancer metastasisK99CA225633 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI GUO, HOUFU · 2018 to 2019
$224k
NCI NIH HHS K99 CA225633NCI NIH HHS R00 CA225633NCI NIH HHS R01 CA105155NCI NIH HHS R01 CA181184NCI NIH HHS R01 CA211125
6 · The paper itself

Abstract

Collagenous stromal accumulations predict a worse clinical outcome in a variety of malignancies. Better tools are needed to elucidate the way in which collagen influences cancer cells. Here, we report a method to generate collagenous matrices that are deficient in key post-translational modifications and evaluate cancer cell behaviors on those matrices. We utilized genetic and biochemical approaches to inhibit lysine hydroxylation and glucosylation on collagen produced by MC-3T3-E1 murine osteoblasts (MC cells). Seeded onto MC cell-derived matrix surface, multicellular aggregates containing lung adenocarcinoma cells alone or in combination with cancer-associated fibroblasts dissociated with temporal and spatial patterns that were influenced by collagen modifications. These findings demonstrate the feasibility of generating defined collagen matrices that are suitable for cell culture studies.

Indexed as

Co-culture modelsCollagenCollagen cross-linksDHLNL, Dehydro-dihydroxylysinonorleucine/its ketoamineER, Endoplasmic ReticulumG, Galactosyl groupGG, Glucosylgalactosyl groupHLCCs, Hydroxylysine aldehyde-derived collagen cross-linksHLNL, Dehydro-hydroxylysinonorleucine/its ketoaminehLys, Helical domain LysineHylald, Aldehide HydroxylysineHyl, HydroxylysineHyp, HydroxyprolineLCC, Lysine aldehyde–derived cross-linksLH, Lysyl hydroxylasesLOX, Lysyl oxidasesLung cancerLysald, Aldehide LysineLys, LysineLysyl hydroxylasesMetastasisPGGHG, Glucosylgalactosylhydroxylysine glucosidasePro, ProlinePTMs, Post-translational modificationstLys, Telopeptidyl Lysine

Identifiers

PMID33543040
PMCPMC7852199
OpenAlexW3089953474

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.