ArticleIranian journal of biotechnology2020
Cytoplasmic Chaperones Enhance Soluble Expression of Anti-EGFR huscFv in
Article in Iranian journal of biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors at 2 institutions in 1 country.
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Abstract
backgroundOverexpression of EGFR is associated with carcinogenesis in more than 70% of head and neck cancers. Anti-EGFR monoclonal antibodies bind to the extracellular domain of EGFR and block the EGFR downstream signaling pathway, which results in the suppression of the growth of the tumor cells.
objectivesIn this study, we attempted to increase the soluble expression of huscfv antibody via co-expression with the cytoplasmic chaperones. MATERIALS AND
methodsTo achieve this purpose, chaperones plasmids pG-KJE8, pGro7, pKjE7, pTf16 and pG-Tf2 encoding cytoplasmic chaperones were co-expressed with the humanized anti-EGFR scFv construct in
resultThe results indicated that the highest concentrations of humanized anti-EGFR scFv were obtained by co-expression of huscFv via chaperone plasmid pG-KJE8 with 0.2 mM concentration of inducer (IPTG), culture temperature of 25 °C, and 4 h incubation time after induction.
conclusionIn conclusion, co-expression with chaperones could be used as an efficient strategy to produce soluble active ScFvs in
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