Evidence map›Paper›PMID 33542475›Full record

ArticleScientific reports2021

Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep.

Hua Yang, Yong-Lin Yang, Guo-Qing Li, Qian Yu, Jinzeng Yang

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Hua YangState Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi, 832000, China.
Yong-Lin YangState Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi, 832000, China.
Guo-Qing LiState Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi, 832000, China.
Qian YuState Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi, 832000, China.
Jinzeng YangDepartment of Human Nutrition, Food and Animal Sciences, University of Hawaii, Honolulu, HI, 96822, USA. jinzeng@hawaii.edu.
Xinjiang Academy of Agricultural and Reclamation Science · CNUniversity of Hawaii System · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aridity and heat are significant environmental stressors that affect sheep adaptation and adaptability, thus influencing immunity, growth, reproduction, production performance, and profitability. The aim of this study was to profile mRNA expression levels in the spleen of indigenous Kazakh sheep breed for comparative analysis with the exotic Suffolk breed. Spleen histomorphology was observed in indigenous Kazakh sheep and exotic Suffolk sheep raised in Xinjiang China. Transcriptome sequencing of spleen tissue from the two breeds were performed via Illumina high-throughput sequencing technology and validated by RT-qPCR. Blood cytokine and IgG levels differed between the two breeds and IgG and IL-1β were significantly higher in Kazakh sheep than in Suffolk sheep (p < 0.05), though spleen tissue morphology was the same. A total of 52.04 Gb clean reads were obtained and the clean reads were assembled into 67,271 unigenes using bioinformatics analysis. Profiling analysis of differential gene expression showed that 1158 differentially expressed genes were found when comparing Suffolk with Kazakh sheep, including 246 up-regulated genes and 912 down-regulated genes. Utilizing gene ontology annotation and pathway analysis, 21 immune- responsive genes were identified as spleen-specific genes associated with adaptive traits and were significantly enriched in hematopoietic cell lineage, natural killer cell-mediated cytotoxicity, complement and coagulation cascades, and in the intestinal immune network for IgA production. Four pathways and up-regulated genes associated with immune responses in indigenous sheep played indispensable and promoting roles in arid and hot environments. Overall, this study provides valuable transcriptome data on the immunological mechanisms related to adaptive traits in indigenous and exotic sheep and offers a foundation for research into adaptive evolution.

Indexed as

Adaptive ImmunityAdaptation, PhysiologicalAnimalsBlood Coagulation FactorsComplement System ProteinsDroughtsErythroid CellsFemaleGene Expression ProfilingGene Expression RegulationGene OntologyHigh-Throughput Nucleotide SequencingHot TemperatureImmunity, InnateImmunoglobulin AImmunoglobulin GBlood Coagulation FactorsComplement System ProteinsImmunoglobulin AImmunoglobulin GInterleukin-1beta

Identifiers

PMID33542475
PMCPMC7862382
OpenAlexW3128982313

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.