ArticleScientific reports2021
Sample pooling as a strategy for community monitoring for SARS-CoV-2.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Maximizing efficiency in sedimentary ancient DNA analysis: a novel extract pooling approach.Scientific reports · 2024Article
- Detection ofInsects · 2024Article
- Underscoring the effect of swab type, workflow, and positive sample order on swab pooling for COVID-19 surveillance testing.Scientific reports · 2023Article
- Pooled sample testing for COVID-19 diagnosis: Evaluation of bi-directional matrix pooling strategies.Journal of virological methods · 2022Article
- Strategies for Scaling up SARS-CoV-2 Molecular Testing Capacity.Clinics in laboratory medicine · 2022Review
- Homebrew: An economical and sensitive glassmilk-based nucleic-acid extraction method for SARS-CoV-2 diagnostics.Cell reports methods · 2022Article
- Molecular accuracy vs antigenic speed: SARS-CoV-2 testing strategies.Current opinion in pharmacology · 2022Review
- Article
- A Regional Pooling Intervention in a High-Throughput COVID-19 Diagnostic Laboratory to Enhance Throughput, Save Resources and Time Over a Period of 6 Months.Frontiers in microbiology · 2022Article
- Identification of Novel Therapeutic Candidates Against SARS-CoV-2 Infections: An Application of RNA Sequencing Toward mRNA Based Nanotherapeutics.Frontiers in microbiology · 2022Article
- Safe and effective pool testing for SARS-CoV-2 detection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2021Article
- Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics.Scientific reports · 2021Article
- Pooling Nasopharyngeal Swab Specimens to Improve Testing Capacity for SARS-CoV-2 by Real-Time RT-PCR.Biological procedures online · 2021Article
- Implementation of Sample Pooling Procedure Using a Rapid SARS-CoV-2 Diagnostic Real-Time PCR Test Performed Prior to Hospital Admission of People with Intellectual Disabilities.International journal of environmental research and public health · 2021Article
- Sample-Pooling Strategy for SARS-CoV-2 Detection among Students and Staff of the University of Sannio.Diagnostics (Basel, Switzerland) · 2021Article
- Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR.Virology journal · 2021Article
- Article
- Pooling of Samples for SARS-CoV-2 Detection Using a Rapid Antigen Test.Frontiers in tropical diseases · 2021Article
- Effectiveness of sample pooling strategies for diagnosis of SARS-CoV-2: Specimen pooling vs. RNA elutes pooling.Indian journal of medical microbiologyArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sample pooling strategy was intended to determine the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2 and process them without significant loss of test usability. Standard molecular diagnostic laboratory equipment, and commercially available centrifugal filters, RNA isolation kits and SARS Cov2 PCR tests were used. The basic idea was to combine and concentrate several samples to the maximal volume, which can be extracted with the single extraction column. Out of 16 tested pools, 12 were positive with cycle threshold (Ct) values within 0.5 and 3.01 Ct of the original individual specimens. The analysis of 112 specimens determined that 12 pools were positive, followed by identification of 6 positive individual specimens among the 112 tested. This testing was accomplished with the use of 16 extractions/PCR tests, resulting in saving of 96 reactions but adding the 40 centrifugal filters. The present study demonstrated that pool testing could detect even up to a single positive sample with Ct value as high as 34. According to the standard protocols, reagents and equipment, this pooling method can be applied easily in current clinical testing laboratories.
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