Evidence map›Paper›PMID 33542253›Full record

ArticleScientific reports2021

Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions.

Min-Dong Chen, Bin Wang, Yong-Ping Li, Mei-Juan Zeng, Jian-Ting Liu, Xin-Ru Ye, Hai-Sheng Zhu, Qing-Fang Wen

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 35 citations in OpenAlex.

  1. qPCR-Based Reference Gene Validation inCurrent issues in molecular biology · 2025
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  6. Reference Genes Selection and Validation forInternational journal of molecular sciences · 2024
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  16. Immunomodulatory Molecular Mechanisms ofJournal of fungi (Basel, Switzerland) · 2022
    Article
  17. Characterization of thePlants (Basel, Switzerland) · 2022
    Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Min-Dong ChenFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China.
Bin WangFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China.
Yong-Ping LiFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China.
Mei-Juan ZengFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China.
Jian-Ting LiuFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China.
Xin-Ru YeFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China.
Hai-Sheng ZhuFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China. zhs0246@163.com.
Qing-Fang WenFujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Vegetable Research Center, Fujian Engineering Research Center for Vegetables, Fuzhou, 350013, Fujian, China. fjvrc@163.com.
Fujian Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Selecting suitable internal reference genes is an important prerequisite for the application of quantitative real-time PCR (qRT-PCR). However, no systematic studies have been conducted on reference genes in luffa. In this study, seven reference genes were selected, and their expression levels in luffa plants exposed to various simulated abiotic stresses [i.e., cold, drought, heat, salt, H

Indexed as

Genes, EssentialAbscisic AcidAdaptation, PhysiologicalCold TemperatureDroughtsGene Expression ProfilingGene Expression Regulation, PlantHot TemperatureHydrogen PeroxideLuffaPeptide Elongation Factor 1Plant ProteinsReal-Time Polymerase Chain ReactionReference StandardsSalinityStress, PhysiologicalAbscisic AcidHydrogen PeroxidePeptide Elongation Factor 1Plant ProteinsSuperoxide Dismutase

Identifiers

PMID33542253
PMCPMC7862638
OpenAlexW3126545649

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.