ArticleLight, science & applications2021
Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector.
Article in Light, science & applications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Spatial triple-correlation spectroscopy reveals heterotrimer dynamics in live cells.Biophysical journal · 2026Article
- Correction of Background in Fluorescence Correlation Spectroscopy for Accurate Determination of Particle Number.Biomolecules · 2025Article
- Fluorescence correlation spectroscopy for particle sizing: A notorious challenge.Biophysical journal · 2025Review
- Single-Photon Single-Particle Tracking.bioRxiv : the preprint server for biology · 2025Article
- Array detection enables large localization range for simple and robust MINFLUX.Light, science & applications · 2025Article
- Wavefront estimation through structured detection in laser scanning microscopy.Biomedical optics express · 2025Article
- The role of RNA in the nanoscale organization of α-synuclein phase separation.NAR molecular medicine · 2025Article
- Spatiotemporal Exciton Tracking with a SPAD Camera.ACS photonics · 2025Article
- Super-resolution microscopy based on the inherent fluctuations of dye molecules.Biomedical optics express · 2025Article
- ALS-associated FUS mutation reshapes the RNA and protein composition of stress granules.Nucleic acids research · 2024Article
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- 4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.Nature communications · 2024Article
- MNature communications · 2024Article
- Single-photon microscopy to study biomolecular condensates.Nature communications · 2023Article
- Silicon-Based Avalanche Photodiodes: Advancements and Applications in Medical Imaging.Nanomaterials (Basel, Switzerland) · 2023Review
- High-resolution single-photon imaging with physics-informed deep learning.Nature communications · 2023Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- The future of bioimage analysis: a dialog between mind and machine.Nature methods · 2023Article
- Focus image scanning microscopy for sharp and gentle super-resolved microscopy.Nature communications · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The combination of confocal laser-scanning microscopy (CLSM) and fluorescence fluctuation spectroscopy (FFS) is a powerful tool in studying fast, sub-resolution biomolecular processes in living cells. A detector array can further enhance CLSM-based FFS techniques, as it allows the simultaneous acquisition of several samples-essentially images-of the CLSM detection volume. However, the detector arrays that have previously been proposed for this purpose require tedious data corrections and preclude the combination of FFS with single-photon techniques, such as fluorescence lifetime imaging. Here, we solve these limitations by integrating a novel single-photon-avalanche-diode (SPAD) array detector in a CLSM system. We validate this new implementation on a series of FFS analyses: spot-variation fluorescence correlation spectroscopy, pair-correlation function analysis, and image-derived mean squared displacement analysis. We predict that the unique combination of spatial and temporal information provided by our detector will make the proposed architecture the method of choice for CLSM-based FFS.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.