ArticleGenome biology2021
3
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Bramble: projection of spliced genomic alignments into transcriptomic space for improved transcript quantification.bioRxiv : the preprint server for biology · 2026Article
- Article
- Regulation of Transcriptional Bursting and Spatial Patterning in EarlybioRxiv : the preprint server for biology · 2025Article
- Conservation assessment of human splice site annotation based on a 470-genome alignment.Nucleic acids research · 2025Article
- Conservation assessment of human splice site annotation based on a 470-genome alignment.bioRxiv : the preprint server for biology · 2025Article
- The HIV-1 Transcriptional Program: From Initiation to Elongation Control.Journal of molecular biology · 2025Review
- Transcriptional bursting: from fundamentals to novel insights.Biochemical Society transactions · 2024Review
- N-terminal tagging of RNA Polymerase II shapes transcriptomes more than C-terminal alterations.iScience · 2024Article
- Review
- Review
- Bayesian inference of polymerase dynamics over the exclusion process.Royal Society open science · 2023Article
- Synergising single-cell resolution and 4sU labelling boosts inference of transcriptional bursting.Genome biology · 2023Article
- A transcriptional cycling model recapitulates chromatin-dependent features of noisy inducible transcription.PLoS computational biology · 2022Article
- A simple guide to de novo transcriptome assembly and annotation.Briefings in bioinformatics · 2022Review
- CFIm25 regulates human stem cell function independently of its role in mRNA alternative polyadenylation.RNA biology · 2022Article
- Anti-bias training for (sc)RNA-seq: experimental and computational approaches to improve precision.Briefings in bioinformatics · 2021Article
- 3Genome biology · 2021Article
- Effects of transcriptional noise on estimates of gene and transcript expression in RNA sequencing experiments.Genome research · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundTranscription in mammalian cells is a complex stochastic process involving shuttling of polymerase between genes and phase-separated liquid condensates. It occurs in bursts, which results in vastly different numbers of an mRNA species in isogenic cell populations. Several factors contributing to transcriptional bursting have been identified, usually classified as intrinsic, in other words local to single genes, or extrinsic, relating to the macroscopic state of the cell. However, some possible contributors have not been explored yet. Here, we focus on processes at the 3
resultsUsing Bayesian methodology, we measure the transcriptional bursting in inducible transgenes, showing that perturbation of polymerase shuttling typically reduces burst size, increases burst frequency, and thus limits transcriptional noise. Analysis based on paired-end tag sequencing (PolII ChIA-PET) suggests that this effect is genome wide. The observed noise patterns are also reproduced by a generative model that captures major characteristics of the polymerase flux between the ends of a gene and a phase-separated compartment.
conclusionsInteractions between the 3
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.