ArticleBMC oral health2021
Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study.
Article in BMC oral health, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Potential of microbial-derived biosurfactants for oral applications-a systematic review.BMC oral health · 2024Pooled it
- Biosurfactants as Antibiofilm Agents for Medical Devices: Mechanisms, Evidence and Integration into Infection Prevention and Control.Microorganisms · 2026Review
- Experimental biofilm models for pharmacokinetic and pharmacodynamic investigations: bridging in vitro, ex vivo and in vivo systems.The Journal of antimicrobial chemotherapy · 2026Review
- Emerging non-antibiotic strategies for implant-associated biofilm infections by reprogramming the dysregulated immune microenvironment.NPJ biofilms and microbiomes · 2026Review
- Bacteriophages Improve the Effectiveness of Rhamnolipids in Combating the Biofilm ofMolecules (Basel, Switzerland) · 2025Article
- Silver multilayer coating on orthopedic implant material of different alloys and surfaces significantly reduces bacterial colonization.Frontiers in cellular and infection microbiology · 2025Article
- Cutting-edge perspectives on biosurfactants: implications for antimicrobial and biomedical applications.3 Biotech · 2024Review
- Exploring Biosurfactants as Antimicrobial Approaches.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Biomaterials science and surface engineering strategies for dental peri-implantitis management.Military Medical Research · 2024Review
- Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity.Scientific reports · 2024Article
- A review on antimicrobial strategies in mitigating biofilm-associated infections on medical implants.Current research in microbial sciences · 2024Review
- Biofouling on titanium implants: a novel formulation of poloxamer and peroxide forRegenerative biomaterials · 2024Article
- Biosurfactant complexed with arginine has antibiofilm activity against methicillin-resistantFuture microbiology · 2024Article
- Therapeutic functions of medical implants from various material categories with integrated biomacromolecular systems.Frontiers in bioengineering and biotechnology · 2024Review
- Mechanical and Biological Properties of Titanium and Its Alloys for Oral Implant with Preparation Techniques: A Review.Materials (Basel, Switzerland) · 2023Review
- Rhamnolipid 89 Biosurfactant Is Effective againstInternational journal of molecular sciences · 2023Article
- In Vitro Models of Bacterial Biofilms: Innovative Tools to Improve Understanding and Treatment of Infections.Nanomaterials (Basel, Switzerland) · 2023Review
- Overview of strategies to improve the antibacterial property of dental implants.Frontiers in bioengineering and biotechnology · 2023Review
- Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity.Applied microbiology and biotechnology · 2022Article
- Antimicrobial and Antibiofilm Coating of Dental Implants-Past and New Perspectives.Antibiotics (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPeri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium.
methodsR89BS was physically adsorbed on titanium discs (TDs). Cytotoxicity of coated TDs was evaluated on normal lung fibroblasts (MRC5) using a lactate dehydrogenase assay. The ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile bacteria was also performed by scanning electron microscopy.
resultsR89BS-coated discs showed no cytotoxic effects. TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 99%, 47% and 7% and of S. epidermidis by 54%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 78% for S. epidermidis at 24 h.
conclusionsR89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.