Evidence map›Paper›PMID 33540739›Full record

ReviewViruses2021

Application of Super-Resolution and Advanced Quantitative Microscopy to the Spatio-Temporal Analysis of Influenza Virus Replication.

Emma Touizer, Christian Sieben, Ricardo Henriques, Mark Marsh, Romain F Laine

Abstract readReview
In one paragraph

Review in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Virus morphology: Insights from super-resolution fluorescence microscopy.Biochimica et biophysica acta. Molecular basis of disease · 2022
    Review
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Emma TouizerDivision of Infection and Immunity, University College London, London WC1E 6AE, UK.ORCID 0000-0002-7164-8555
Christian SiebenDepartment of Cell Biology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.ORCID 0000-0002-2836-2623
Ricardo HenriquesMRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK.ORCID 0000-0002-2043-5234
Mark MarshMRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK.ORCID 0000-0002-0213-3259
Romain F LaineMRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK.ORCID 0000-0002-2151-4487

Funding

Medical Research Council MC_U12266BMedical Research Council MC_UU_00012/1Medical Research Council MC_UU00012/1Medical Research Council MR/K015826/1Medical Research Council MR/N013867/1Medical Research Council MR/T027924/1Wellcome Trust 203276/Z/16/Z
6 · The paper itself

Abstract

With an estimated three to five million human cases annually and the potential to infect domestic and wild animal populations, influenza viruses are one of the greatest health and economic burdens to our society, and pose an ongoing threat of large-scale pandemics. Despite our knowledge of many important aspects of influenza virus biology, there is still much to learn about how influenza viruses replicate in infected cells, for instance, how they use entry receptors or exploit host cell trafficking pathways. These gaps in our knowledge are due, in part, to the difficulty of directly observing viruses in living cells. In recent years, advances in light microscopy, including super-resolution microscopy and single-molecule imaging, have enabled many viral replication steps to be visualised dynamically in living cells. In particular, the ability to track single virions and their components, in real time, now allows specific pathways to be interrogated, providing new insights to various aspects of the virus-host cell interaction. In this review, we discuss how state-of-the-art imaging technologies, notably quantitative live-cell and super-resolution microscopy, are providing new nanoscale and molecular insights into influenza virus replication and revealing new opportunities for developing antiviral strategies.

Indexed as

AnimalsHumansInfluenza, HumanMicroscopyOrthomyxoviridaeSpatio-Temporal AnalysisVirus Replicationadvanced light microscopyexpansion microscopyinfluenza viruslive-cell microscopyquantitative microscopySIMSMLMSTEDSTORMsuper-resolution microscopyviral replication

Identifiers

PMID33540739
PMCPMC7912985

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.