Evidence map›Paper›PMID 33540582›Full record

ArticleInternational journal of molecular sciences2021

Biosensor-Based Directed Evolution of Methanol Dehydrogenase from

Thien-Kim Le, Su-Bin Ju, Hye-Won Lee, Jin-Young Lee, So-Hyung Oh, Kil-Koang Kwon, Bong-Hyun Sung, Seung-Goo Lee, Soo-Jin Yeom

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 26 citations in OpenAlex.

  1. Comparative Genomic Analysis and Data About the Metabolism of the GenusInternational journal of molecular sciences · 2026
    Article
  2. Review
  3. Article
  4. Article
  5. Cobalt modulates methanol turnover of the alcohol dehydrogenase inApplied and environmental microbiology · 2025
    Article
  6. Review
  7. Article
  8. Article
  9. Biosensor development for single-cell detection of glucuronate.Journal of industrial microbiology & biotechnology · 2023
    Article
  10. Journal of fungi (Basel, Switzerland) · 2022
    Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Methanol Dehydrogenases as a Key Biocatalysts for Synthetic Methylotrophy.Frontiers in bioengineering and biotechnology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Thien-Kim LeSchool of Biological Sciences and Biotechnology, Graduate School, Chonnam National University, Yongbong-ro 77, Gwangju 61186, Korea.ORCID 0000-0001-5239-4943
Su-Bin JuSchool of Biological Sciences and Biotechnology, Graduate School, Chonnam National University, Yongbong-ro 77, Gwangju 61186, Korea.
Hye-Won LeeSynthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.ORCID 0000-0003-2255-3418
Jin-Young LeeSynthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
So-Hyung OhSynthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
Kil-Koang KwonSynthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
Bong-Hyun SungSynthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
Seung-Goo LeeSynthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.ORCID 0000-0003-4539-9812
Soo-Jin YeomSchool of Biological Sciences and Biotechnology, Graduate School, Chonnam National University, Yongbong-ro 77, Gwangju 61186, Korea.ORCID 0000-0001-7307-237X
Korea Research Institute of Bioscience and Biotechnology · KRChonnam National University · KR

Funding

the C1 Gas Refinery Program funded by the Ministry of Science and ICT 2018M3D3A1A01055732the C1 Gas Refinery Program funded by the Ministry of Science and ICT 2018M3D3A1A01056181
6 · The paper itself

Abstract

Methanol dehydrogenase (Mdh), is a crucial enzyme for utilizing methane and methanol as carbon and energy sources in methylotrophy and synthetic methylotrophy. Engineering of Mdh, especially NAD-dependent Mdh, has thus been actively investigated to enhance methanol conversion. However, its poor catalytic activity and low methanol affinity limit its wider application. In this study, we applied a transcriptional factor-based biosensor for the direct evolution of Mdh from

Indexed as

MutationAlcohol OxidoreductasesBacillaceaeBacterial ProteinsBiosensing TechniquesKineticsMethanolalcohol dehydrogenase (acceptor)Alcohol OxidoreductasesBacterial ProteinsMethanolbiosensorLysinibacillus xylanilyticusmethanol dehydrogenasescreeningsynthetic methylotrophy

Identifiers

PMID33540582
PMCPMC7867188
OpenAlexW3127473421

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.