ArticleJournal of experimental zoology. Part B, Molecular and developmental evolution2021
A star is born again: Methods for larval rearing of an emerging model organism, the False clownfish Amphiprion ocellaris.
Article in Journal of experimental zoology. Part B, Molecular and developmental evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Cell-cell communication as underlying principle governing color pattern formation in teleost fishes.Nature communications · 2026Article
- Comparative transcriptomics reveal the common anteroposterior molecular blueprint of adult bilaterian guts.PLoS biology · 2026Article
- Article
- Ultraviolet B radiation impairs coral reef fish development.Biology open · 2025Article
- Anemonefish use sialic acid metabolism as Trojan horse to avoid giant sea anemone stinging.BMC biology · 2025Article
- Anemonefishes: A model system for evolutionary genomics.F1000Research · 2023Review
- The real Nemo movie: Description of embryonic development in Amphiprion ocellaris from first division to hatching.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Review
- A star is born again: Methods for larval rearing of an emerging model organism, the False clownfish Amphiprion ocellaris.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021Article
- CRISPR/Cas9-mediated generation of biallelic F0 anemonefish (Amphiprion ocellaris) mutants.PloS one · 2021Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As interest increases in ecological, evolutionary, and developmental biology (Eco-Evo-Devo), wild species are increasingly used as experimental models. However, we are still lacking a suitable model for marine fish species, as well as coral reef fishes that can be reared at laboratory scales. Extensive knowledge of the life cycle of anemonefishes, and the peculiarities of their biology, make them relevant marine fish models for developmental biology, ecology, and evolutionary sciences. Here, we present standard methods to maintain breeding pairs of the anemonefish Amphiprion ocellaris in captivity, obtain regular good quality spawning, and protocols to ensure larval survival throughout rearing. We provide a detailed description of the anemonefish husbandry system and life prey culturing protocols. Finally, a "low-volume" rearing protocol useful for the pharmacological treatment of larvae is presented. Such methods are important as strict requirements for large volumes in rearing tanks often inhibit continuous treatments with expensive or rare compounds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.