ArticleCytometry. Part B, Clinical cytometry2021
Validation of inducible basophil biomarkers: Time, temperature and transportation.
Article in Cytometry. Part B, Clinical cytometry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Treatment Approaches for Omalizumab-Refractory Chronic Spontaneous Urticaria.Journal of inflammation research · 2026Review
- Basophil Activation Test in IgE-Mediated Wheat Allergy: Diagnostic and Clinical Applications-A Narrative Review.Diagnostics (Basel, Switzerland) · 2025Review
- A hand-operated microfluidic sample preparation-to-analysis workflow for simplifying the basophil activation test.Lab on a chip · 2025Article
- Enhancing allergy diagnosis: mass spectrometry as a complementary technique to the basophil activation test.Frontiers in allergy · 2025Review
- A diagnostic approach to IgE-mediated food allergy: A practical algorithm.Journal of food allergy · 2024Article
- Towards standardizing basophil identification by flow cytometry.Frontiers in allergy · 2023Article
- Towards an FDA-cleared basophil activation test.Frontiers in allergy · 2022Review
- Validation of inducible basophil biomarkers: Time, temperature and transportation.Cytometry. Part B, Clinical cytometry · 2021Article
- Basophil activation test in food allergy: is it ready for real-time?Current opinion in allergy and clinical immunology · 2021Review
- The Basophil Activation Test for Clinical Management of Food Allergies: Recent Advances and Future Directions.Journal of asthma and allergy · 2021Review
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Authors and funding
31 authors.
Funding
Abstract
backgroundThe short stability window of several hours from blood collection to measuring basophil activation has limited the use of flow cytometry-based basophil activation assays in clinical settings. We examine if it is possible to extend this window to 1 day allowing for shipment of samples between laboratories. Several options exist for reporting the results including reporting all the measured values directly, calculating ratios and reporting a single value covering all measured results. Each of these options have different stability and value to the physician.
methodsWhole blood samples from peanut allergic patients were stimulated with four different peanut concentrations at Day 0, Day 1, and Day 2. Samples were stored under temperature-controlled conditions. Flow cytometry was used to analyze the samples. The basophil activation and degranulation were measured as percentage of positive CD63 basophils and CD203c MFI fold change. Shipped samples were transported under ambient conditions.
resultsThe results show that CD63 is a stable marker at Day 1. The CD203c ratio decreases significantly at Day 1. Calculating the CD63/IgE ratio proves to be more stable than CD63 alone. The most stable readouts are the semi-quantitative results and the trajectory of the dose response curve. Finally, we confirmed that the stability can be extended to samples shipped overnight to the laboratory.
conclusionsIt is possible to extend the stability of the basophil activation assay to 1 day for samples stored at 18-25°C as well as samples shipped under ambient conditions as long as the temperature is within the 2-37°C range.
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