ArticlePlant biotechnology journal2021
Jasmonate- and abscisic acid-activated AaGSW1-AaTCP15/AaORA transcriptional cascade promotes artemisinin biosynthesis in Artemisia annua.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- A Systematic Review of Alternative Artemisinin Production Strategies.International journal of molecular sciences · 2025Pooled it
- AaSnRK2.6 interacts with AaORA to positively regulate artemisinin biosynthesis inHorticulture research · 2026Article
- Review
- Regulatory architecture of high-altitude adaptation in Artemisia: from signal perception to specialized metabolism.Plant cell reports · 2026Review
- The Role of Long Non-Coding RNA in Atherosclerosis: Mechanism and Intervention of Traditional Chinese Medicine.International journal of molecular sciences · 2026Review
- Genome-Wide Identification of WRKY Gene Family in Artemisia and Its Expression Analysis of Aphid Resistance.International journal of molecular sciences · 2026Article
- Jasmonate-Activated AaWRKY9-AabHLH93/AabHLH93-AaMYB7 Complexes Balance Artemisinin Biosynthesis in Artemisia annua.Plant biotechnology journal · 2026Article
- The Jasmonate-Responsive SmMPK3-SmWRKY33 Module Positively Regulates Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2026Article
- Comparative transcriptome analysis reveals abscisic acid-induced bHLH transcription factors involved in saikosaponin biosynthesis inPlant signaling & behavior · 2025Article
- Regulation of important natural products biosynthesis by WRKY transcription factors in plants.Journal of advanced research · 2025Review
- Single-nucleus transcriptomics reveal the morphogenesis and artemisinin biosynthesis in Artemisia annua glandular trichomes.Nature communications · 2025Article
- Metabolic Engineering of Terpenoid Biosynthesis in Medicinal Plants: From Genomic Insights to Biotechnological Applications.Current issues in molecular biology · 2025Review
- Molecular Decoding of Phytohormone Crosstalk: JA-Mediated Key Regulatory Nodes and Signal Integration.Plants (Basel, Switzerland) · 2025Review
- The Dynamic Changes in Biosynthesis and Spatiotemporal Distribution of Phytohormones Under Jasmonic Acid Treatment Provide Insights into Hormonal Regulation inPlants (Basel, Switzerland) · 2025Article
- AaMYC3 bridges the regulation of glandular trichome density and artemisinin biosynthesis in Artemisia annua.Plant biotechnology journal · 2025Article
- Unraveling TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR Transcription Factors in Safflower: A Blueprint for Stress Resilience and Metabolic Regulation.Molecules (Basel, Switzerland) · 2025Article
- A transcription factor of SHI family AaSHI1 activates artemisinin biosynthesis genes in Artemisia annua.BMC genomics · 2024Article
- Regulation of Glandular Size and Phytoalexin Biosynthesis by a Negative Feedback Loop in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Advanced metabolic engineering strategies for increasing artemisinin yield inHorticulture research · 2024Article
- Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments inInternational journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 2 institutions in 1 country.
Funding
Abstract
Artemisinin, a sesquiterpene lactone widely used in malaria treatment, was discovered in the medicinal plant Artemisia annua. The biosynthesis of artemisinin is efficiently regulated by jasmonate (JA) and abscisic acid (ABA) via regulatory factors. However, the mechanisms linking JA and ABA signalling with artemisinin biosynthesis through an associated regulatory network of downstream transcription factors (TFs) remain enigmatic. Here we report AaTCP15, a JA and ABA dual-responsive teosinte branched1/cycloidea/proliferating (TCP) TF, which is essential for JA and ABA-induced artemisinin biosynthesis by directly binding to and activating the promoters of DBR2 and ALDH1, two genes encoding enzymes for artemisinin biosynthesis. Furthermore, AaORA, another positive regulator of artemisinin biosynthesis responds to JA and ABA, interacts with and enhances the transactivation activity of AaTCP15 and simultaneously activates AaTCP15 transcripts. Hence, they form an AaORA-AaTCP15 module to synergistically activate DBR2, a crucial gene for artemisinin biosynthesis. More importantly, AaTCP15 expression is activated by the multiple reported JA and ABA-responsive TFs that promote artemisinin biosynthesis. Among them, AaGSW1 acts at the nexus of JA and ABA signalling to activate the artemisinin biosynthetic pathway and directly binds to and activates the AaTCP15 promoter apart from the AaORA promoter, which further facilitates formation of the AaGSW1-AaTCP15/AaORA regulatory module to integrate JA and ABA-mediated artemisinin biosynthesis. Our results establish a multilayer regulatory network of the AaGSW1-AaTCP15/AaORA module to regulate artemisinin biosynthesis through JA and ABA signalling, and provide an interesting avenue for future research exploring the special transcriptional regulation module of TCP genes associated with specialized metabolites in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.