ArticleReproduction (Cambridge, England)2021
Equine maternal aging affects oocyte lipid content, metabolic function and developmental potential.
Article in Reproduction (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed.
- Impact of dietary essential fatty acids on phospholipid composition and mitochondrial function in aged mares.Scientific reports · 2025Article
- Mitochondria-driven inflammation: a new frontier in ovarian ageing.Journal of translational medicine · 2025Review
- Mitochondrial dysfunction in oocytes: implications for fertility and ageing.Journal of ovarian research · 2025Review
- Delayed blastocyst development is associated with altered metabolism and proteome in male and female bovine embryos†.Biology of reproduction · 2025Article
- Follicular metabolic dysfunction, oocyte aneuploidy and ovarian aging: a review.Journal of ovarian research · 2025Review
- Comparison of the Mitophagy and Apoptosis Related Gene Expressions in Waste Embryo Culture Medium of Female Infertility Types.Life (Basel, Switzerland) · 2024Article
- Fluctuations of antimüllerian hormone, ovarian follicular reserve, and antioxidant status throughout the estrous cycle in aged mares.Open veterinary journal · 2024Article
- Ovarian aging: energy metabolism of oocytes.Journal of ovarian research · 2024Review
- Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation.Scientific reports · 2024Article
- Mapping the lipidomic secretome of the early equine embryo.Frontiers in veterinary science · 2024Article
- In vitro fertilization program in white rhinoceros.Reproduction (Cambridge, England) · 2023Article
- Influence of Intrauterine Fluid Detection, Number of Transfers and Age of the Recipient on Pregnancy Rate and Early Embryonic Loss in a Commercial Embryo Transfer Program.Animals : an open access journal from MDPI · 2023Article
- Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points.Frontiers in cell and developmental biology · 2023Article
- Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares.Frontiers in cell and developmental biology · 2023Article
- Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass.BMC genomics · 2022Article
- Oocyte metabolic function, lipid composition, and developmental potential are altered by diet in older mares.Reproduction (Cambridge, England) · 2022Article
- Novel Synthetic oviductal fluid for Conventional Freezing 1 (SCF1) culture medium improves development and cryotolerance of in vitro produced Holstein embryos.Journal of animal science · 2022Article
- Article
Corrections and comments
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Authors and funding
6 authors.
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Abstract
Advanced maternal age is associated with a decline in fertility and oocyte quality. We used novel metabolic microsensors to assess effects of mare age on single oocyte and embryo metabolic function, which has not yet been similarly investigated in mammalian species. We hypothesized that equine maternal aging affects the metabolic function of oocytes and in vitro-produced early embryos, oocyte mitochondrial DNA (mtDNA) copy number, and relative abundance of metabolites involved in energy metabolism in oocytes and cumulus cells. Samples were collected from preovulatory follicles from young (≤14 years) and old (≥20 years) mares. Relative abundance of metabolites in metaphase II oocytes (MII) and their respective cumulus cells, detected by liquid and gas chromatography coupled to mass spectrometry, revealed that free fatty acids were less abundant in oocytes and more abundant in cumulus cells from old vs young mares. Quantification of aerobic and anaerobic metabolism, respectively measured as oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in a microchamber containing oxygen and pH microsensors, demonstrated reduced metabolic function and capacity in oocytes and day-2 embryos originating from oocytes of old when compared to young mares. In mature oocytes, mtDNA was quantified by real-time PCR and was not different between the age groups and not indicative of mitochondrial function. Significantly more sperm-injected oocytes from young than old mares resulted in blastocysts. Our results demonstrate a decline in oocyte and embryo metabolic activity that potentially contributes to the impaired developmental competence and fertility in aged females.
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