Evidence map›Paper›PMID 33538948›Full record

ArticleJournal of biomolecular NMR2021

A simple and cost-effective protocol for high-yield expression of deuterated and selectively isoleucine/leucine/valine methyl protonated proteins in Escherichia coli grown in shaker flasks.

Mengli Cai, Ying Huang, John Lloyd, Robert Craigie, G Marius Clore

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Investigating Complexin-Membrane Interactions Using NMR and Optical Methods.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mengli Cai *Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0520, USA.
Ying Huang *Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0540, USA.
John LloydLaboratory of Biorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0820, USA.
Robert CraigieLaboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0540, USA.
G Marius CloreLaboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0520, USA. mariusc@mail.nih.gov.ORCID http://orcid.org/0000-0003-3809-1027

Funding

Studies of nucleoprotein complexes involved in retroviral DNA integrationZIADK036169 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI CRAIGIE, ROBERT · 2009 to 2025
$22.5M
NMR STUDIES OF THREE-DIMENSIONAL STRUCTURE OF MACROMOLECULES IN SOLUTIONZ01DK029023 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI CLORE, G. MARIUS · 1988 to 2008
$949k
Studies of nucleoprotein complexes involved in retroviral DNA integrationZ01DK036169 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI CRAIGIE, ROBERT · 2007 to 2008
$852k
Intramural NIH HHS Z01 DK029023Intramural NIH HHS Z01 DK036169NIDDK NIH HHS DK029023
6 · The paper itself

Abstract

A simple and cost-effective protocol is presented for expression of perdeuterated, Ile/Leu/Val

Indexed as

Cost-Benefit AnalysisProtonsAmino AcidsBiochemistryBioreactorsDeuteriumEscherichia coliEscherichia coli ProteinsGene ExpressionIsoleucineLeucineValineAmino AcidsDeuteriumEscherichia coli ProteinsIsoleucineLeucineProtonsValineHigh cell-density inductionM9 ++ mediumNMRPerdeuterated and methyl protonated proteinsProtein expressionShaker flask

Identifiers

PMID33538948
PMCPMC8855621

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.