Evidence map›Paper›PMID 33538877›Full record

ArticleApplied microbiology and biotechnology2021

Comparative proteomic analyses reveal the metabolic aspects and biotechnological potential of nitrate assimilation in the yeast Dekkera bruxellensis.

Irina Charlot Peña-Moreno, Denise Castro Parente, Karolini Miranda da Silva, Elton Pedro Nunes Pena, Fabiana Aparecida Cavalcante Silva, Tercilio Calsa Junior, Will de Barros Pita, Marcos Antonio de Morais

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In one paragraph

Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Irina Charlot Peña-MorenoLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil.
Denise Castro ParenteLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil.
Karolini Miranda da SilvaLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil.
Elton Pedro Nunes PenaLaboratory of Plant Genomics and Proteomics, Department of Genetics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil.
Fabiana Aparecida Cavalcante SilvaLaboratory of Plant Genomics and Proteomics, Department of Genetics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil.
Tercilio Calsa JuniorLaboratory of Plant Genomics and Proteomics, Department of Genetics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil.
Will de Barros PitaLaboratory of Bioprocesses, Department of Antibiotics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil.
Marcos Antonio de MoraisLaboratory of Microbial Genetics, Department of Genetics, Federal University of Pernambuco, Recife, PE, 50760-901, Brazil. marcos.moraisjr@ufpe.br.ORCID https://orcid.org/0000-0002-9727-0300
Universidade Federal de Pernambuco · BR

Funding

CNPq 303551/2017-8CNPq 409767/2018-2FACEPE 1452-2.01/10
6 · The paper itself

Abstract

The yeast Dekkera bruxellensis is well-known for its adaptation to industrial ethanol fermentation processes, which can be further improved if nitrate is present in the substrate. To date, the assimilation of nitrate has been considered inefficient because of the apparent energy cost imposed on cell metabolism. Recent research, however, has shown that nitrate promotes growth rate and ethanol yield when oxygen is absent from the environment. Given this, the present work aimed to identify the biological mechanisms behind this physiological behaviour. Proteomic analyses comparing four contrasting growth conditions gave some clues on how nitrate could be used as primary nitrogen source by D. bruxellensis GDB 248 (URM 8346) cells in anaerobiosis. The superior anaerobic growth in nitrate seems to be a consequence of increased cell metabolism (glycolytic pathway, production of ATP and NADPH and anaplerotic reactions providing metabolic intermediates) regulated by balanced activation of TORC1 and NCR de-repression mechanisms. On the other hand, the poor growth observed in aerobiosis is likely due to an oxidative stress triggered by nitrate when oxygen is present. These results represent a milestone regarding the knowledge about nitrate metabolism and might be explored for future use of D. bruxellensis as an industrial yeast. KEY POINTS: • Nitrate can be regarded as preferential nitrogen source for D. bruxellensis. • Oxidative stress limits the growth of D. bruxellensis in nitrate in aerobiosis. • Nitrate is a nutrient for novel industrial bioprocesses using D. bruxellensis.

Indexed as

DekkeraBrettanomycesFermentationNitratesProteomicsNitratesEthanol fermentationMetabolic regulationNitrogen metabolismOxidative stressProteomicsYeast growth

Identifiers

PMID33538877
OpenAlexW3127940558

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.