ArticleiScience2021
Establishment of murine gut microbiota in gnotobiotic mice.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Fecal microbiota transplantation from psychiatric patients to mice - systematic review of methodologies and a call for standardization.Translational psychiatry · 2026Pooled it
- From association to causation: a decision-aware framework for reproducible biomarker discovery and precision intervention design in the human gut microbiome.Briefings in bioinformatics · 2026Article
- Probiotic-conditioned microbiota from preterm infants modulate immune response to pathogen challenge in a microbiota-humanized mouse model.Frontiers in immunology · 2026Article
- The gut microbiome is associated with susceptibility to febrile malaria in Malian children.Nature communications · 2024Article
- Host or the Hosted? Effects of Non-Nutritive Sweeteners on Intestinal and Microbial Mechanisms of Glycemic Control.Nutrients · 2024Article
- Comparative Metagenomic Analysis of Bacteriophages and Prophages in Gnotobiotic Mouse Models.Microorganisms · 2024Article
- Intestinal persistence of Bifidobacterium infantis is determined by interaction of host genetics and antibiotic exposure.The ISME journal · 2024Article
- Engraftment of aging-related human gut microbiota and the effect of a seven-species consortium in a pre-clinical model.Gut microbes · 2023Article
- The Impact of Long-Term Macrolide Exposure on the Gut Microbiome and Its Implications for Metabolic Control.Microbiology spectrum · 2023Article
- The gut microbiome and mental health: advances in research and emerging priorities.Molecular psychiatry · 2022Review
- Faecal microbiota transplant ameliorates gut dysbiosis and cognitive deficits in Huntington's disease mice.Brain communications · 2022Article
- Gut microbiota transplantation for colonization of germ-free mice.STAR protocols · 2021Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determining whether associations between gut microbiota characteristics and host physiology represent causal relationships is a fundamental challenge for microbiome research. We report a detailed investigation of microbiome assembly in C57BL/6 germ-free mice across a period of 70 days and compare the effects of single and multiple rounds of gavage, using both native and antibiotic-disrupted murine donor material. Recipients of the native microbiota did not achieve compositional stability until day 28 and persistent differences to donor microbiota remained until day 70. Performing multiple rounds of gavage significantly increased the cumulative number of detected taxa (mean increase: 10.4%) and compositional similarity to donor, and significantly reduced within-group variance (p < 0.05). Multiple rounds of gavage with antibiotic-disrupted microbiota provided no substantial benefit in relation to compositional similarity to donor or within-group variance. The process of donor microbiota establishment in recipient animals is necessary before experimentation commences and is considerably influenced by donor microbiota characteristics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.