ArticleFrontiers in physiology2020
Effects of Simulated Microgravity on Ultrastructure and Apoptosis of Choroidal Vascular Endothelial Cells.
Article in Frontiers in physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- L-NAME promotes foam cell formation and synthetic phenotype marker changes in vascular smooth muscle cells independent of hemodynamic effects.BMC molecular and cell biology · 2026Article
- AstroOphthalmology: Basic and Pathophysiological Concepts of Space-Induced Ocular Changes.Eye and brain · 2026Review
- PI3K/AKT/mTOR pathway and c-Myc inhibit hepatocyte suspension under simulated microgravity.NPJ microgravity · 2025Article
- The Heart in Space: Effects of Microgravity on Different Cell Types and Their Functions in the Cardiovascular System.Biomedicines · 2025Review
- Aging and Altered Gravity: A Cellular Perspective.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- SIRT5 Alleviates Apoptosis of Vascular Endothelial Cells Under Simulated Microgravity via Desuccinylation of ERO1A.International journal of molecular sciences · 2025Article
- Cardiovascular adaptations and pathological changes induced by spaceflight: from cellular mechanisms to organ-level impacts.Military Medical Research · 2024Review
- Impact of Microgravity and Other Spaceflight Factors on Retina of Vertebrates and Humans In Vivo and In Vitro.Life (Basel, Switzerland) · 2023Review
- Review
- Choroidal circulation disturbance is an initial factor in outer retinal degeneration in rats under simulated weightlessness.Frontiers in physiology · 2023Article
- Evaluation of the Effects of Microgravity on Activated Primary Human Hepatic Stellate Cells.International journal of molecular sciences · 2022Article
- Detrimental effects of simulated microgravity on mast cell homeostasis and function.Frontiers in immunology · 2022Article
- PINK1-Dependent Mitophagy Reduced Endothelial Hyperpermeability and Cell Migration Capacity Under Simulated Microgravity.Frontiers in cell and developmental biology · 2022Article
- Microgravity and space radiation inhibit autophagy in human capillary endothelial cells, through either opposite or synergistic effects on specific molecular pathways.Cellular and molecular life sciences : CMLS · 2021Article
- Genomic Changes Driven by Radiation-Induced DNA Damage and Microgravity in Human Cells.International journal of molecular sciences · 2021Review
- Role of Apoptosis in Wound Healing and Apoptosis Alterations in Microgravity.Frontiers in bioengineering and biotechnology · 2021Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt was confirmed that simulated microgravity (SMG) led to ultrastructural alterations and apoptosis in many types of microvascular endothelial cells. However, whether SMG would also affect choroidal vascular endothelial cells (CVECs) remains unknown. This study was designed to investigate the effects of SMG on ultrastructure and apoptosis of CVECs.
methodsThe rotary cell culture system (RCCS) was utilized to simulate microgravity condition. Human CVECs were cultured under normal gravity (NG) or SMG condition for 3 days. The ultrastructure was viewed under transmission electron microscopy, and the organization of F-actin was observed by immunofluorescence staining. Additionally, the apoptosis percentage was calculated using flow cytometry. Moreover, the mRNA and protein expression of BAX, Bcl-2, Caspase3, Cytochrome C, p-AKT, and p-PI3K were detected with quantitative PCR and Western blot at different exposure time.
resultsIn the SMG group, CVECs presented with a shrunk cell body, chromatin condensation and margination, mitochondria vacuolization, and apoptotic bodies. The amount of F-actin decreased, and the filaments of F-actin were sparse or even partly discontinuous after cultivation under SMG for 72 h. The proportions of apoptotic CVECs in SMG groups at 24 and 72 h were significantly higher than those in the NG group (
conclusionSimulated microgravity conditions can lead the alterations of the F-actin structure and apoptosis of CVECs. The Bcl-2 apoptosis pathway and PI3K/AKT pathway may participate in the damage of CVECs caused by SMG.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.