ArticleScientific reports2021
Primer evaluation and development of a droplet digital PCR protocol targeting amoA genes for the quantification of Comammox in lakes.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Depth-dependent distribution patterns of ammonia- and nitrite-oxidizing microorganisms in the water column of stratified lakes.Scientific reports · 2025Article
- Comprehensive evaluation of primer pairs targeting the ammonia monooxygenase subunit A gene of complete ammonia-oxidizingMicrobiology spectrum · 2024Article
- Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings.Diagnostics (Basel, Switzerland) · 2024Review
- Vertical Distribution and Seasonal Patterns of Candidatus Nitrotoga in a Sub-Alpine Lake.Microbes and environments · 2024Article
- Ecophysiological and Genomic Characterization of the Freshwater Complete Ammonia OxidizerApplied and environmental microbiology · 2023Article
- A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of ViableMicroorganisms · 2022Article
- Evaluation of four primer sets for analysis of comammox communities in black soils.Frontiers in microbiology · 2022Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, little is known about the ecological significance of Comammox (COMplete AMMonia OXidizers) Nitrospira in the water column of freshwater lakes. Water samples collected along depth profiles were used to investigate the distribution of Comammox in 13 lakes characterized by a wide range of physicochemical properties. Several published primers, which target the α-subunit of the ammonia monooxygenase, generated non-specific PCR products or did not amplify target genes from lake water and other habitats. Therefore, a new primer set has been designed for specific detection of Comammox in lakes. The high specificity of the PCR assay was confirmed by sequencing analysis. Quantification of Comammox amoA genes in lake water samples based on droplet digital PCR (ddPCR) revealed very low abundances (not exceeding 85 amoA copies ml
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