ArticleScience advances2021
Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- An Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides Modifies the Fecal Microbiome and Metabolome in Infants with Cow's Milk Protein Allergy.International journal of molecular sciences · 2023Trial
- Dietary and nutritional strategies for reducing disease risk associated with antibiotic-induced dysbiosis.Gut microbes · 2026Review
- Nanocellulose Alleviates Intrahepatic Cholestasis of Pregnancy via Gut Microbiota-Mediated Bile Acid Homeostasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Gut microbial bile salt hydrolase as a metabolic gatekeeper in digestive homeostasis and disease.Frontiers in immunology · 2026Review
- Fetal programming by the parental microbiome of offspring behavior, and DNA methylation and gene expression within the hippocampus.Microbiome · 2025Article
- A Screening Assay for Bile Acid-Transforming Microorganisms Using Engineered Bacterial Biosensors.Biosensors · 2025Article
- Probiotic acoustic biosensors for noninvasive imaging of gut inflammation.Nature communications · 2025Article
- Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases.Cell chemical biology · 2025Article
- Classifying compounds as prebiotics - scientific perspectives and recommendations.Nature reviews. Gastroenterology & hepatology · 2025Review
- New insights into microbial bile salt hydrolases: from physiological roles to potential applications.Frontiers in microbiology · 2025Review
- Probing and manipulating the gut microbiome with chemistry and chemical tools.Gut microbiome (Cambridge, England) · 2025Review
- Bile salt hydrolase: a key player in gut microbiota and its implications for metabolic dysfunction-associated steatotic liver disease.Microbiome research reports · 2025Review
- Bacterial imaging in tumour diagnosis.Microbial biotechnology · 2024Review
- Bile Salt Hydrolase Activity-Based Probes for Monitoring Gut Microbial Bile Acid Metabolism.Chembiochem : a European journal of chemical biology · 2024Article
- Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases.bioRxiv : the preprint server for biology · 2024Article
- Emerging Issues and Initial Insights into Bacterial Biofilms: From Orthopedic Infection to Metabolomics.Antibiotics (Basel, Switzerland) · 2024Review
- Role of Hydrogen Sulfide in Inflammatory Bowel Disease.Antioxidants (Basel, Switzerland) · 2023Review
- Profiling rhythmicity of bile salt hydrolase activity in the gut lumen with a rapid fluorescence assay.Organic & biomolecular chemistry · 2023Article
- In vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter.Nature communications · 2023Article
- Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice.Science advances · 2023Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used for quick and cost-effective quantification of BSH activity across a broad range of biological settings including pure enzymes and bacteria, intact fecal slurries, and noninvasive imaging in live animals, as well as for the assessment of BSH activity in the entire gastrointestinal tract of mice and humans. Using this assay, we showed that certain types of prebiotics are capable of increasing BSH activity of the gut microbiota in vivo and successfully demonstrated potential application of this assay as a noninvasive diagnostic test to predict the clinical status of inflammatory bowel disease (IBD) patients.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.