ArticleParasitology2021
Dual RNA-Seq transcriptome analysis of chicken macrophage-like cells (HD11) infected
Article in Parasitology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Advances in Poultry RNA-Omics Research: Technologies, RNA Information Layers, and Applications in Complex Traits.Animals : an open access journal from MDPI · 2026Review
- Transcriptomic Analysis of Domestic Ducks' Proventriculus Infected withVeterinary sciences · 2026Article
- HDAC1 and SATB1 positively regulate immune responses in chicken macrophages.Poultry science · 2026Article
- Advancing immunity and disease resistance in chickens through genome editing.Journal of animal science and biotechnology · 2026Review
- UnderstandingFrontiers in cellular and infection microbiology · 2026Review
- Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage.BMC veterinary research · 2025Article
- Study of dense granule proteins inFrontiers in immunology · 2025Article
- A novel avian intestinal epithelial cell line: its characterization and exploration as an in vitro infection culture model for Eimeria species.Parasites & vectors · 2024Article
- Article
- Molecular characterization of cDNA coding for 33.5 and 41 kDa oocyst and sporocyst proteins that are differentially regulated in different strains ofFrontiers in veterinary science · 2024Article
- Microbiota promotes recruitment and pro-inflammatory response of caecal macrophages during E. tenella infection.Gut pathogens · 2023Article
- The role of CaPoultry science · 2022Article
- Transcriptional changes in LMH cells induced byFrontiers in veterinary science · 2022Article
- Time-series transcriptome analysis identified differentially expressed genes in broiler chicken infected with mixedFrontiers in genetics · 2022Article
- Dual RNA-seq transcriptome analysis of caecal tissue during primary Eimeria tenella infection in chickens.BMC genomics · 2021Article
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Authors and funding
7 authors.
Funding
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Abstract
The study aimed to monitor parasite and host gene expression during the early stages of Eimeria tenella infection of chicken cells using dual RNA-Seq analysis. For this, we used chicken macrophage-like cell line HD11 cultures infected in vitro with purified E. tenella sporozoites. Cultures were harvested between 2 and 72 h post-infection and mRNA was extracted and sequenced. Dual RNA-Seq analysis showed clear patterns of altered expression for both parasite and host genes during infection. For example, genes in the chicken immune system showed upregulation early (2–4 h), a strong downregulation of genes across the immune system at 24 h and a repetition of early patterns at 72 h, indicating that invasion by a second generation of parasites was occurring. The observed downregulation may be due to immune self-regulation or to immune evasive mechanisms exerted by E. tenella. Results also suggested pathogen recognition receptors involved in E. tenella innate recognition, MRC2, TLR15 and NLRC5 and showed distinct chemokine and cytokine induction patterns. Moreover, the expression of several functional categories of Eimeria genes, such as rhoptry kinase genes and microneme genes, were also examined, showing distinctive differences which were expressed in sporozoites and merozoites.
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