ArticleStem cell research & therapy2021
Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation.
Article in Stem cell research & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Biological Functions and Applications of Exosomes from Periodontal Ligament Stem Cells.Proteomes · 2026Review
- 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells.Cell death & disease · 2026Article
- Osteogenic Differentiation of Mesenchymal Stem Cells Induced by Geometric Mechanotransductive 3D-Printed Poly-(L)-Lactic Acid Matrices.International journal of molecular sciences · 2025Article
- Systematic evaluation of normalization approaches in tandem mass tag and label-free protein quantification data using PRONE.Briefings in bioinformatics · 2025Article
- Silencing FOXA1 suppresses inflammation caused by LPS and promotes osteogenic differentiation of periodontal ligament stem cells through the TLR4/MyD88/NF-κB pathway.Biomolecules & biomedicine · 2025Article
- Impact of increasingly complex cell culture conditions on the proteome of human periodontal ligament stem cells.Regenerative medicine · 2025Article
- Naringenin modulates the NO‑cGMP‑PKG signaling pathway by binding to AKT to enhance osteogenic differentiation in hPDLSCs.International journal of molecular medicine · 2024Article
- Proanthocyanidins Ameliorate LPS-Inhibited Osteogenesis of PDLSCs by Restoring Lysine Lactylation.International journal of molecular sciences · 2024Article
- Mitochondrial Dysfunction in the Pathogenesis and Treatment of Oral Inflammatory Diseases.International journal of molecular sciences · 2023Review
- Effects of vitamin A in promoting proliferation and osteogenic differentiation of human periodontal ligament cells.In vitro cellular & developmental biology. Animal · 2023Article
- Transcriptome landscape comparison of periodontium in developmental and renewal stages.Frontiers in endocrinology · 2023Article
- Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering.Histochemistry and cell biology · 2022Article
- A Review of In Vivo and Clinical Studies Applying Scaffolds and Cell Sheet Technology for Periodontal Ligament Regeneration.Biomolecules · 2022Review
- NMR Metabolomics Assessment of Osteogenic Differentiation of Adipose-Tissue-Derived Mesenchymal Stem Cells.Journal of proteome research · 2022Article
- Future Drug Targets in Periodontal Personalised Medicine-A Narrative Review.Journal of personalized medicine · 2022Review
- Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine.Stem cell research & therapy · 2022Review
- Comprehensive analysis of the long noncoding RNA-associated competitive endogenous RNA network in the osteogenic differentiation of periodontal ligament stem cells.BMC genomics · 2022Article
- Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2022Article
- Meta-analysis of proteomics data from osteoblasts, bone, and blood: Insights into druggable targets, active factors, and potential biomarkers for bone biomaterial design.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman periodontal ligament stem cells (hPDLSCs) are ideal seed cells for periodontal regeneration. A greater understanding of the dynamic protein profiles during osteogenic differentiation contributed to the improvement of periodontal regeneration tissue engineering.
methodsTandem Mass Tag quantitative proteomics was utilized to reveal the temporal protein expression pattern during osteogenic differentiation of hPDLSCs on days 0, 3, 7 and 14. Differentially expressed proteins (DEPs) were clustered and functional annotated by Gene Ontology (GO) terms. Pathway enrichment analysis was performed based on the Kyoto Encyclopedia of Genes and Genomes database, followed by the predicted activation using Ingenuity Pathway Analysis software. Interaction networks of redox-sensitive signalling pathways and oxidative phosphorylation (OXPHOS) were conducted and the hub protein SOD2 was validated with western blotting.
resultsA total of 1024 DEPs were identified and clustered in 5 distinctive clusters representing dynamic tendencies. The GO enrichment results indicated that proteins with different tendencies show different functions. Pathway enrichment analysis found that OXPHOS was significantly involved, which further predicted continuous activation. Redox-sensitive signalling pathways with dynamic activation status showed associations with OXPHOS to various degrees, especially the sirtuin signalling pathway. SOD2, an important component of the sirtuin pathway, displays a persistent increase during osteogenesis. Data are available via ProteomeXchange with identifier PXD020908.
conclusionThis is the first in-depth dynamic proteomic analysis of osteogenic differentiation of hPDLSCs. It demonstrated a dynamic regulatory mechanism of hPDLSC osteogenesis and might provide a new perspective for research on periodontal regeneration.
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