ArticleBioTechniques2021
Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA.
Article in BioTechniques, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Laboratory-based molecular test alternatives to RT-PCR for the diagnosis of SARS-CoV-2 infection.The Cochrane database of systematic reviews · 2024Pooled it
- A Novel Voltammetric Point-of-Care Device for Rapid and Accurate Nucleic Acid and Viral Pathogen Detection.ACS omega · 2026Article
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of High-Burden Viral Diseases in Resource-Limited Countries.Pathogens (Basel, Switzerland) · 2026Review
- Heat-actuated valve implementation in a point-of-care, paper-based microfluidic device for infectious disease detection.PloS one · 2026Article
- Point-of-need one-pot multiplexed RT-LAMP test for detecting three common respiratory viruses in saliva.Biosensors & bioelectronics · 2025Article
- Fast-Flu: RT-RPA-CRISPR/Cas12a assisted one-step platform for rapid influenza B virus detection.Microbiology spectrum · 2025Article
- Development of Dual 'RT-LAMP-LFA' Rapid Detection Technology With Gold Magnetic Nanoparticles for Influenza Virus.Microbial biotechnology · 2025Article
- RUNCOV: a one-pot triplex real-time RT-LAMP as a point-of-care diagnostic tool for detecting SARS-CoV-2.Biology methods & protocols · 2025Article
- Advancements in LAMP-Based Diagnostics: Emerging Techniques and Applications in Viral Detection with a Focus on Herpesviruses in Transplant Patient Management.International journal of molecular sciences · 2024Review
- Deep Learning Enabled Universal Multiplexed Fluorescence Detection for Point-of-Care Applications.ACS sensors · 2024Article
- Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection.Diagnostics (Basel, Switzerland) · 2023Review
- Clinical Validation of a Colorimetric Loop-Mediated Isothermal Amplification Using a Portable Device for the Rapid Detection of SARS-CoV-2.Diagnostics (Basel, Switzerland) · 2023Article
- Article
- Comparison of Reverse Transcriptase (RT) Activities of Various M-MuLV RTs for RT-LAMP Assays.Biology · 2022Article
- An isothermal lab-on-phone test for easy molecular diagnosis of SARS-CoV-2 near patients and in less than 1 hour.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022Article
- Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses.Analytica chimica acta · 2022Review
- Article
- Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid.Chembiochem : a European journal of chemical biology · 2022Article
- Monolithic, 3D-printed lab-on-disc platform for multiplexed molecular detection of SARS-CoV-2.Sensors and actuators. B, Chemical · 2022Article
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The ongoing pandemic has demonstrated the utility of widespread surveillance and diagnostic detection of the novel SARS-CoV-2. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) has enabled broader testing, but current LAMP tests only detect single targets and require separate reactions for controls. With flu season in the Northern Hemisphere, the ability to screen for multiple targets will be increasingly important, and the ability to include internal controls in RT-LAMP allows for improved efficiency. Here we describe multiplexed RT-LAMP with four targets (SARS-CoV-2, influenza A, influenza B, human RNA) in a single reaction using real-time and end point fluorescence detection. Such increased functionality of RT-LAMP will enable even broader adoption of this molecular testing approach and aid in the fight against this public health threat.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.