ArticlePloS one2021
Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- Biomarker candidates for oocyte and embryo quality assessment in assisted reproduction: A systematic review.JBRA assisted reproduction · 2025Pooled it
- Follicle Size Regulates Cytoplasmic Maturation, Lipid Content, and Embryo Cryosurvival in Bovine Oocytes Matured In-Vitro.Molecular reproduction and development · 2026Article
- The Critical Functions of FGF2, LIF and IGF1 in the Improvement of In Vitro Embryo Production.Biomolecules · 2026Review
- Insulin-like growth factor 1 as a key paracrine mediator in mesenchymal stem cell-based intervention for ovarian ageing: a review.Journal of ovarian research · 2026Review
- Lower Omega-6-Omega-3 Ratio Increased Milk Production and Had Limited Effects on Early Pregnancy Development in Dairy Cattle.Animals : an open access journal from MDPI · 2026Article
- Cytokine supplementation influences bovine embryo transcriptome during the preimplantation period.Reproduction & fertility · 2025Article
- Short- and long-term effects of uterine disease on oocyte developmental capacity in postpartum dairy cows.Theriogenology · 2025Article
- Human Recombinant Interleukin-6 and Leukemia Inhibitory Factor Improve Inner Cell Mass Cell Number but Lack Cryoprotective Activities on In Vitro-Produced Bovine Blastocysts.Animals : an open access journal from MDPI · 2025Article
- Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates.Scientific reports · 2025Article
- Molecular factors driving the development of bovine embryos and embryo-like structures.Animal reproduction · 2025Review
- Unraveling the complexity of follicular fluid: insights into its composition, function, and clinical implications.Journal of ovarian research · 2024Review
- Effect of the addition of IGF-1 during in vitro culture on the embryonic development speed from different crossbreed bovine embryos.Tropical animal health and production · 2024Article
- Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro.Scientific reports · 2024Article
- Effects of Melatonin, GM-CSF, IGF-1, and LIF in Culture Media on Embryonic Development: Potential Benefits of Individualization.International journal of molecular sciences · 2024Review
- Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency.Animals : an open access journal from MDPI · 2023Article
- Improved pregnancy rate and sex ratio in fresh/frozenJournal of animal science and technology · 2023Article
- Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle.Animals : an open access journal from MDPI · 2023Review
- Bovine blastocyst-like structures derived from stem cell cultures.Cell stem cell · 2023Article
- Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero.Reproduction & fertility · 2023Article
- Combination of CNP, MT and FLI during IVM Significantly Improved the Quality and Development Abilities of Bovine Oocytes and IVF-Derived Embryos.Antioxidants (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro embryo production systems are limited by their inability to consistently produce embryos with the competency to develop to the blastocyst stage, survive cryopreservation, and establish a pregnancy. Previous work identified a combination of three cytokines [fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1)], called FLI, that we hypothesize improve preimplantation development of bovine embryos in vitro. To test this hypothesis, FLI was supplemented into oocyte maturation or embryo culture medium. Embryos were produced in vitro using abattoir-derived oocytes and fertilized with sperm from a single bull known to have high fertility. After an 18-20 h fertilization period, putative zygotes were cultured in synthetic oviductal fluid (SOF) for 8 days. The addition of FLI to the oocyte maturation medium increased (P < 0.05) the dissociation of transzonal projections at 12, 18, and 24 h of maturation, as well as, the proportion of oocytes that reached the metaphase II stage of meiosis. Additionally, lipid content was decreased (P < 0.05) in the blastocyst stage embryo. The addition of FLI during the culture period increased development to the blastocyst stage, cytoskeleton integrity, and survival following slow freezing, as well as, decreased post thaw cell apoptosis (P < 0.05). In conclusion, the supplementation of these cytokines in vitro has the potential to alleviate some of the challenges associated with the cryo-survival of in vitro produced bovine embryos through improving embryo development and embryo quality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.