ArticlePloS one2021
Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples.STAR protocols · 2025Article
- Detection and molecular characterisation of subtype B avian metapneumovirus in commercial chickens and co-infection with bacteria pathogens in Nigeria.Tropical animal health and production · 2025Article
- Article
- Silica-coated magnetic particles for efficient RNA extraction for SARS-CoV-2 detection.Heliyon · 2024Article
- A high-performance polymer composite column for coronavirus nucleic acid purification.Scientific reports · 2024Article
- An Open One-Step RT-qPCR for SARS-CoV-2 detection.PloS one · 2024Article
- The quality of commercial SARS-CoV-2 nucleic acid tests in Ecuador: lessons from COVID-19 pandemic for advancing social equity through microbiology.Frontiers in cellular and infection microbiology · 2023Article
- Graph data science and machine learning for the detection of COVID-19 infection from symptoms.PeerJ. Computer science · 2023Article
- CRISPR-based point-of-care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS-CoV-2 detection.Journal of biochemical and molecular toxicology · 2022Review
- Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests.Viruses · 2022Review
- Open access methods and protocols promote open science in a pandemic.STAR protocols · 2022Article
- Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays.Microbial cell (Graz, Austria) · 2022Article
- SARS-CoV-2 Diagnostics Based on Nucleic Acids Amplification: From Fundamental Concepts to Applications and Beyond.Frontiers in cellular and infection microbiology · 2022Review
- UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection.STAR protocols · 2021Article
- A low-cost and open-source protocol to produce key enzymes for molecular detection assays.STAR protocols · 2021Article
- Unlocking SARS-CoV-2 detection in low- and middle-income countries.Cell reports methods · 2021Article
- Article
- Simple, Inexpensive RNA Isolation and One-Step RT-qPCR Methods for SARS-CoV-2 Detection and General Use.Current protocols · 2021Article
- Point-of-Care Diagnostic Tools for Surveillance of SARS-CoV-2 Infections.Frontiers in public health · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Re-opening of communities in the midst of the ongoing COVID-19 pandemic has ignited new waves of infections in many places around the world. Mitigating the risk of reopening will require widespread SARS-CoV-2 testing, which would be greatly facilitated by simple, rapid, and inexpensive testing methods. This study evaluates several protocols for RNA extraction and RT-qPCR that are simpler and less expensive than prevailing methods. First, isopropanol precipitation is shown to provide an effective means of RNA extraction from nasopharyngeal (NP) swab samples. Second, direct addition of NP swab samples to RT-qPCRs is evaluated without an RNA extraction step. A simple, inexpensive swab collection solution suitable for direct addition is validated using contrived swab samples. Third, an open-source master mix for RT-qPCR is described that permits detection of viral RNA in NP swab samples with a limit of detection of approximately 50 RNA copies per reaction. Quantification cycle (Cq) values for purified RNA from 30 known positive clinical samples showed a strong correlation (r2 = 0.98) between this homemade master mix and commercial TaqPath master mix. Lastly, end-point fluorescence imaging is found to provide an accurate diagnostic readout without requiring a qPCR thermocycler. Adoption of these simple, open-source methods has the potential to reduce the time and expense of COVID-19 testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.