Evidence map›Paper›PMID 33534551›Full record

ArticleAnalytical chemistry2021

Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies.

Frøydis Sved Skottvoll, Frederik André Hansen, Sean Harrison, Ida Sneis Boger, Ago Mrsa, Magnus Saed Restan, Matthias Stein, Elsa Lundanes, Stig Pedersen-Bjergaard, Aleksandra Aizenshtadt and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 3 countries.

Frøydis Sved SkottvollDepartment of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway.ORCID 0000-0002-5299-2502
Frederik André HansenDepartment of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway.ORCID 0000-0002-1666-0447
Sean HarrisonHybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway.
Ida Sneis BogerDepartment of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway.
Ago MrsaDepartment of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway.
Magnus Saed RestanDepartment of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway.
Matthias SteinInstitute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethovenstr. 55, DE-38106 Braunschweig, Germany.
Elsa LundanesDepartment of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway.
Stig Pedersen-BjergaardDepartment of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, NO-0316 Oslo, Norway.ORCID 0000-0002-1666-8043
Aleksandra AizenshtadtHybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway.
Stefan KraussHybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway.
Gareth SullivanHybrid Technology Hub-Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1112, Blindern, NO-0317 Oslo, Norway.
Inger Lise BogenSection for Drug Abuse Research, Department of Forensic Sciences, Oslo University Hospital, P.O. Box 4950, Nydalen, NO-0424 Oslo, Norway.
Steven Ray WilsonDepartment of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway.ORCID 0000-0002-9755-1188
University of Oslo · NOOslo University Hospital · NOTechnische Universität Braunschweig · DEUniversity of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multiwell EME (parallel-EME) holding 100 μL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements.

Indexed as

OrganoidsPharmaceutical PreparationsLiverMass SpectrometryMembranes, ArtificialMembranes, ArtificialPharmaceutical Preparations

Identifiers

PMID33534551
PMCPMC8023518
OpenAlexW3127235137

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.