Evidence map›Paper›PMID 33533815›Full record

ArticleRevista do Instituto de Medicina Tropical de Sao Paulo2021

Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR.

João Augusto Franco Leonel, Bruna Tannihão, Julia Assis Arantes, Geovanna Vioti, Julia Cristina Benassi, Roberta Ariboni Brandi, Helena Lage Ferreira, Lara Borges Keid, Rodrigo Martins Soares, Trícia Maria Ferreira de Sousa Oliveira

Open access · diamondAbstract read
In one paragraph

Article in Revista do Instituto de Medicina Tropical de Sao Paulo, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it, 6 citations in OpenAlex.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

João Augusto Franco LeonelUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Laboratório de Medicina Veterinária Preventiva Aplicada, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0003-0617-301X
Bruna TannihãoUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Laboratório de Medicina Veterinária Preventiva Aplicada, Pirassununga, São Paulo, Brazil.
Julia Assis ArantesUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Programa de Pós-Graduação em Biociência Animal, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-6900-6182
Geovanna ViotiUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Laboratório de Medicina Veterinária Preventiva Aplicada, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0001-7762-5453
Julia Cristina BenassiUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Laboratório de Medicina Veterinária Preventiva Aplicada, Pirassununga, São Paulo, Brazil.
Roberta Ariboni BrandiUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Zootecnia, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0003-4044-7408
Helena Lage FerreiraUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Laboratório de Medicina Veterinária Preventiva Aplicada, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-3261-3712
Lara Borges KeidUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Laboratório de Medicina Veterinária Preventiva Aplicada, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0003-3212-596X
Rodrigo Martins SoaresUniversidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Programa de Pós-Graduação em Epidemiologia Experimental Aplicada às Zoonoses, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-7736-7914
Trícia Maria Ferreira de Sousa OliveiraUniversidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Medicina Veterinária, Laboratório de Medicina Veterinária Preventiva Aplicada, Pirassununga, São Paulo, Brazil.ORCID http://orcid.org/0000-0003-3916-3600
Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Visceral leishmaniasis (VL) is a neglected tropical disease caused by the Leishmania infantum parasite. The protozoan is able to infect several domestic and wild mammals. Since the first report on Leishmania spp. infection in horses in South America, leishmaniasis in equids has been highlighted in Brazil. A molecular epidemiological survey was carried out to verify the occurrence of Leishmania spp. DNA in horses and donkeys, in leishmaniases endemic areas in Sao Paulo State, Brazil. To this end, blood samples were obtained from 107 horses and 36 donkeys and subjected to DNA extraction followed by PCR targeting the ITS-1 region. Among the horses and donkeys, 1.87% (2/107) and 8.33% (3/36) were positive by PCR, respectively. The DNA sequencing of the ITS-1 amplification products confirmed L. infantum DNA in these animals. Our results suggest that horses and donkeys from non-VL and VL endemic areas of São Paulo State may be infected by the parasite.

Indexed as

AnimalsBrazilDNAEquidaeHorsesLeishmania infantumLeishmaniasis, VisceralPolymerase Chain ReactionDNA

Identifiers

PMID33533815
PMCPMC7845934
OpenAlexW3127304194

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.