ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2021
Optical clearing reveals TNBS-induced morphological changes of VGLUT2-positive nerve fibers in mouse colorectum.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Colorectum and embedded networks of nerve fibers present auxetic responses during uniaxial circumferential extension.Acta biomaterialia · 2026Article
- Suppression of visceral nociception by C-fiber transmission block using temporal interference sinusoidal stimulation.Pain reports · 2026Article
- Methods for quantitative analyses of nerve fiber deformation in the myenteric plexus under loading of mouse distal colon and rectum.Medical engineering & physics · 2025Article
- Pharmacological Targeting of Hyperpolarization-Activated Cyclic Nucleotide-Gated Cation Channels on Bladder Afferent Sensory Transmission.Lower urinary tract symptoms · 2025Article
- Bidirectional regulation of the brain-gut-microbiota axis following traumatic brain injury.Neural regeneration research · 2025Article
- Neural representation of cytokines by vagal sensory neurons.Nature communications · 2025Article
- Understanding mechanotransduction in the distal colon and rectum via multiscale and multimodal computational modeling.Journal of the mechanical behavior of biomedical materials · 2024Article
- Morphological, molecular, and functional characterization of mouse glutamatergic myenteric neurons.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Sex differences in zymosan-induced behavioral visceral hypersensitivity and colorectal afferent sensitization.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Predicting the micromechanics of embedded nerve fibers using a novel three-layered model of mouse distal colon and rectum.Journal of the mechanical behavior of biomedical materials · 2022Article
- Toward Elucidating the Physiological Impacts of Residual Stresses in the Colorectum.Journal of biomechanical engineering · 2022Article
- The gut, its microbiome, and the brain: connections and communications.The Journal of clinical investigation · 2021Review
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Colorectal hypersensitivity and sensitization of both mechanosensitive and mechanically insensitive afferents develop after intracolonic instillation of 2,4,6-trinitrobenzenesulfonic acid (TNBS) in the mouse, a model of postinfectious irritable bowel syndrome. In mice in which ∼80% of extrinsic colorectal afferents were labeled genetically using the promotor for vesicular glutamate transporter type 2 (VGLUT2), we systematically quantified the morphology of VGLUT2-positive axons in mouse colorectum 7-28 days following intracolonic TNBS treatment. After removal, the colorectum was distended (20 mmHg), fixed with paraformaldehyde, and optically cleared to image VGLUT2-positive axons throughout the colorectal wall thickness. We conducted vector path tracing of individual axons to allow systematic quantification of nerve fiber density and shape. Abundant VGLUT2-positive nerve fibers were present in most layers of the colorectum, except the serosal and longitudinal muscular layers. A small percentage of VGLUT2-positive myenteric plexus neurons was also detected. Intracolonic TNBS treatment significantly reduced the number of VGLUT2-positive nerve fibers in submucosal, myenteric plexus, and mucosal layers at
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