Evidence map›Paper›PMID 33532784›Full record

ArticlemedRxiv : the preprint server for health sciences2021

SARS-CoV-2 recruits a haem metabolite to evade antibody immunity.

Annachiara Rosa, Valerie E Pye, Carl Graham, Luke Muir, Jeffrey Seow, Kevin W Ng, Nicola J Cook, Chloe Rees-Spear, Eleanor Parker, Mariana Silva Dos Santos and 30 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 20 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

40 authors at 9 institutions in 4 countries.

Annachiara RosaChromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
Valerie E PyeChromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
Carl GrahamDepartment of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, UK.
Luke MuirInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London, UK.
Jeffrey SeowDepartment of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, UK.
Kevin W NgRetroviral Immunology Laboratory, The Francis Crick Institute, London, UK.
Nicola J CookChromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
Chloe Rees-SpearInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London, UK.
Eleanor ParkerDepartment of Infectious Disease, St-Mary's Campus, Imperial College London, UK.
Mariana Silva Dos SantosMetabolomics Science Technology Platform, The Francis Crick Institute, London, UK.
Carolina RosadasDepartment of Infectious Disease, St-Mary's Campus, Imperial College London, UK.
Alberto SusanaDepartment of Cellular, Computational and Integrative Biology, University of Trento, Italy.
Hefin RhysFlow Cytometry Science and Technology Platform, The Francis Crick Institute, London, UK.
Andrea NansStructural Biology Science Technology Platform, The Francis Crick Institute, London, UK.
Laura MasinoStructural Biology Science Technology Platform, The Francis Crick Institute, London, UK.
Chloe RoustanStructural Biology Science Technology Platform, The Francis Crick Institute, London, UK.
Evangelos ChristodoulouStructural Biology Science Technology Platform, The Francis Crick Institute, London, UK.
Rachel UlfertsCell Biology of Infection Laboratory, The Francis Crick Institute, London, UK.
Antoni WrobelStructural Biology of Disease Processes Laboratory, The Francis Crick Institute, London, UK.
Charlotte-Eve ShortDepartment of Infectious Disease, St-Mary's Campus, Imperial College London, UK.
Michael FertlemanCutrale Perioperative & Ageing Group, Imperial College London, UK.
Rogier W SandersDepartment of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam, Netherlands.
Judith HeaneyAdvanced Pathogen Diagnostic Unit, University College London Hospitals NHS Foundation Trust, London, UK.
Moira SpyerAdvanced Pathogen Diagnostic Unit, University College London Hospitals NHS Foundation Trust, London, UK.
Svend KjærStructural Biology Science Technology Platform, The Francis Crick Institute, London, UK.
Andy RiddellFlow Cytometry Science and Technology Platform, The Francis Crick Institute, London, UK.
Michael H MalimDepartment of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, UK.
Rupert BealeCell Biology of Infection Laboratory, The Francis Crick Institute, London, UK.
James I MacRaeMetabolomics Science Technology Platform, The Francis Crick Institute, London, UK.
Graham P TaylorDepartment of Infectious Disease, St-Mary's Campus, Imperial College London, UK.
Eleni NastouliAdvanced Pathogen Diagnostic Unit, University College London Hospitals NHS Foundation Trust, London, UK.
Marit J van GilsDepartment of Medical Microbiology, Amsterdam UMC, University of Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam, Netherlands.
Peter B RosenthalStructural Biology of Cells and Viruses Laboratory, The Francis Crick Institute, London, UK.
Massimo PizzatoDepartment of Cellular, Computational and Integrative Biology, University of Trento, Italy.
Myra O McClureDepartment of Infectious Disease, St-Mary's Campus, Imperial College London, UK.
Richard S TedderDepartment of Infectious Disease, St-Mary's Campus, Imperial College London, UK.
George KassiotisRetroviral Immunology Laboratory, The Francis Crick Institute, London, UK.
Laura E McCoyInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London, UK.
Katie J DooresDepartment of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London, UK.
Peter CherepanovChromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
The Francis Crick Institute · GBSt Mary's Hospital · GBKing's College London · GBUniversity College London · GBUniversity College London Hospitals NHS Foundation Trust · GBUniversity of Trento · ITAmsterdam University Medical Centers · NLCornell University · USImperial College London · GB

Funding

Technology Development 2: MAS NMR and dynamic nuclear polarization for HIV-1 structural biologyP50AI150481 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GRONENBORN, ANGELA M. · 2019 to 2021
$14.1M
Medical Research Council MC_PC_19078Medical Research Council MC_PC_19082Medical Research Council MR/R008698/1NIAID NIH HHS P50 AI150481Wellcome Trust 102898/B/13/Z
6 · The paper itself

Abstract

The coronaviral spike is the dominant viral antigen and the target of neutralizing antibodies. We show that SARS-CoV-2 spike binds biliverdin and bilirubin, the tetrapyrrole products of haem metabolism, with nanomolar affinity. Using cryo-electron microscopy and X-ray crystallography we mapped the tetrapyrrole interaction pocket to a deep cleft on the spike N-terminal domain (NTD). At physiological concentrations, biliverdin significantly dampened the reactivity of SARS-CoV-2 spike with immune sera and inhibited a subset of neutralizing antibodies. Access to the tetrapyrrole-sensitive epitope is gated by a flexible loop on the distal face of the NTD. Accompanied by profound conformational changes in the NTD, antibody binding requires relocation of the gating loop, which folds into the cleft vacated by the metabolite. Our results indicate that the virus co-opts the haem metabolite for the evasion of humoral immunity via allosteric shielding of a sensitive epitope and demonstrate the remarkable structural plasticity of the NTD.

Identifiers

PMID33532784
PMCPMC7852234
OpenAlexW3121812492

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.