Evidence map›Paper›PMID 33532741›Full record

ArticleSTAR protocols2021

Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force microscopy.

Amlan Barai, Alakesh Das, Shamik Sen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Amlan BaraiDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Alakesh DasDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Shamik SenDepartment of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Indian Institute of Technology Bombay · INWeizmann Institute of Science · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantification of nuclear stiffness is challenging for cells encapsulated within a 3D extracellular matrix (ECM). Here, we describe an experimental setup for measuring microenvironment-dependent tuning of nuclear stiffness using an atomic force microscope (AFM). In our setup, ECM-coated polyacrylamide hydrogels mimic the stiffness of the microenvironment, enabling the measurement of nuclear stiffness using an AFM probe in live cancer cells. For complete details on the use and execution of this protocol, please refer to Das et al. (2019) (https://doi.org/10.1016/j.matbio.2019.01.001).

Indexed as

Cell NucleusExtracellular MatrixMicroscopy, Atomic ForceNeoplasmsTumor MicroenvironmentAcrylic ResinsCell Line, TumorHumansAcrylic Resinspolyacrylamide gelsAtomic force microscopy (AFM)BiophysicsCancerCell biology

Identifiers

PMID33532741
PMCPMC7829340
OpenAlexW3124863003

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.