ArticleScientific reports2021
Environmental (e)RNA advances the reliability of eDNA by predicting its age.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Establishing Practical and Cost-Effective Methodologies for Preservation and Extraction of Environmental RNA (eRNA) From Aquatic Samples.Molecular ecology resources · 2026Article
- Biomonitoring 3.0: Beyond Taxa Lists to Evidence-Tiered Monitoring of Ecosystem Dynamics and Interactions.Ecology and evolution · 2026Review
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- Environmental RNA reflects seasonal changes in the metabolic activity of stream salamanders.Scientific reports · 2026Article
- Residual eDNA in eRNA Extracts Skews eRNA-Based Biodiversity Assessment: Call for Optimised DNase Treatment.Molecular ecology resources · 2026Article
- Environmental DNA/RNA for non-invasive early detection and monitoring of pathogen dynamics in Atlantic salmon (Aquaculture (Amsterdam, Netherlands) · 2026Article
- Pattern of Detections Across Multiple Environmental Messenger RNAs (e-mRNAs) in Stressor-Exposed Zebrafish (Ecology and evolution · 2026Article
- From individuals to communities: How genomics is transforming biodiversity conservation.Genetics and molecular biology · 2026Article
- Environmental DNA as a tool for hydropower impact assessments: current status, special considerations, and future integration.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Article
- Environmental RNA-Based Metatranscriptomics as a Novel Biomonitoring Tool: A Case Study of Glyphosate-Based Herbicide Effects on Freshwater Eukaryotic Communities.Molecular ecology · 2025Article
- Comparative Decay Dynamics and Detectability of eDNA and eRNA in Connected and Isolated Freshwater Mesocosms Using Digital PCR.Molecular ecology resources · 2025Article
- Extracellular rRNA Profiling Reveals the Sinking and Cell Lysis Dynamics of Marine Microeukaryotes.Environmental microbiology · 2025Article
- Differential decay of multiple environmental nucleic acid components.Scientific reports · 2025Article
- Environmental transcriptomics under heat stress: Can environmental RNA reveal changes in gene expression of aquatic organisms?Molecular ecology · 2025Article
- Keystone active bacterial lineages associated with Penaeus stylirostris shrimp health across larvae stages.PloS one · 2025Article
- Stain-free artificial intelligence-assisted light microscopy for the identification of leukocyte morphology change in presence of bacteria.Frontiers in bioinformatics · 2025Article
- Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health.Molecular biotechnology · 2024Review
- Evaluating eDNA and eRNA metabarcoding for aquatic biodiversity assessment: From bacteria to vertebrates.Environmental science and ecotechnology · 2024Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA (eDNA) analysis has advanced conservation biology and biodiversity management. However, accurate estimation of age and origin of eDNA is complicated by particle transport and the presence of legacy genetic material, which can obscure accurate interpretation of eDNA detection and quantification. To understand the state of genomic material within the environment, we investigated the degradation relationships between (a) size of fragments (long vs short), (b) genomic origins (mitochondrial vs nuclear), (c) nucleic acids (eDNA vs eRNA), and (d) RNA types (messenger (m)RNA vs ribosomal (r)RNA) from non-indigenous Dreissena mussels. Initial concentrations of eRNA followed expected transcriptional trends, with rRNAs found at > 1000 × that of eDNA, and a mitosis-associated mRNA falling below detection limits within 24 h. Furthermore, the ratio of eRNA:eDNA significantly decreased throughout degradation, potentially providing an estimate for the age of genomic material. Thus, eRNA quantification can increase detection due to the high concentrations of rRNAs. Furthermore, it may improve interpretation of positive detections through the eRNA:eDNA ratio and/or by detecting low abundant mitosis-associated mRNAs that degrade within ~ 24 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.