ArticleStructure (London, England : 1993)2021
Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin binding.
Article in Structure (London, England : 1993), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Microtubule Inner Protein CFAP77 Contributes to Sperm Motility and Male Fertility in Mice.Andrology · 2026Article
- In Silico Discovery of Potential Inhibitors Targeting the MEIG1-PACRG Complex for Male Contraceptive Development.Proteins · 2025Article
- Review
- SARS-CoV-2 infection results in a unique lung proteome long after virus resolution in the hamster.Npj viruses · 2024Article
- GametesOmics: A Comprehensive Multi-omics Database for Exploring the Gametogenesis in Humans and Mice.Genomics, proteomics & bioinformatics · 2024Article
- Article
- Doublet microtubule inner junction protein FAP20 recruits tubulin to the microtubule lattice.Structure (London, England : 1993) · 2023Article
- FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction.Nature communications · 2023Article
- Modulation of inner junction proteins contributes to axoneme differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Structurally divergent and recurrently mutated regions of primate genomes.bioRxiv : the preprint server for biology · 2023Article
- The inner junction protein CFAP20 functions in motile and non-motile cilia and is critical for vision.Nature communications · 2022Article
- TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse.Scientific reports · 2022Article
- Ependymal cells and neurodegenerative disease: outcomes of compromised ependymal barrier function.Brain communications · 2022Review
- Single-cell analysis of gene expression in the substantia nigra pars compacta of a pesticide-induced mouse model of Parkinson's disease.Translational neuroscience · 2022Article
- Functional Expression, Purification and Identification of Interaction Partners of PACRG.Molecules (Basel, Switzerland) · 2021Article
- Article
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Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
Abstract
The Parkin co-regulated gene protein (PACRG) binds at the inner junction between doublet microtubules of the axoneme, a structure found in flagella and cilia. PACRG binds to the adaptor protein meiosis expressed gene 1 (MEIG1), but how they bind to microtubules is unknown. Here, we report the crystal structure of human PACRG in complex with MEIG1. PACRG adopts a helical repeat fold with a loop that interacts with MEIG1. Using the structure of the axonemal doublet microtubule from the protozoan Chlamydomonas reinhardtii and single-molecule fluorescence microscopy, we propose that PACRG binds to microtubules while simultaneously recruiting free tubulin to catalyze formation of the inner junction. We show that the homologous PACRG-like protein also mediates dual tubulin interactions but does not bind MEIG1. Our findings establish a framework to assess the function of the PACRG family of proteins and MEIG1 in regulating axoneme assembly.
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