ArticlePloS one2021
Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Real-Time PCR Assays for Quantification of Trypanosoma cruzi in Food Samples: A Tool for Surveillance of Oral Chagas Disease Outbreaks.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system.BMC microbiology · 2025Article
- The Development of a One-Step RT-qPCR for the Detection and Quantification of Viable Forms ofInternational journal of molecular sciences · 2024Article
- Parasite Burden ofMicroorganisms · 2024Article
- Validation of the NAT Chagas IVD Kit for the Detection and Quantification ofLife (Basel, Switzerland) · 2023Article
- Comparative Assessment of Two Commercial Real-Time PCR Assays for the Diagnosis ofMicroorganisms · 2023Article
- Comparison of the Influence of Different Nucleic Acid Extraction Assays on the Sensitivity ofMicroorganisms · 2022Article
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Authors and funding
7 authors.
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Abstract
In Brazil, orally acquired T. cruzi infection has become the most relevant transmission mechanisms from public health perspective. Around 70% of new Chagas disease cases have been associated with consumption of contaminated food or beverages. Açai (Euterpe oleracea and Euterpe precatoria) is currently one of the most commercialized Amazonian fruits in the Brazilian and international markets. Therefore, it has become important to incorporate in the production process some procedures to measure out effective hygiene and product quality control required by global market. Molecular methods have been developed for rapid detection and quantification of T. cruzi DNA in several biological samples, including food matrices, for epidemiological investigation of Chagas disease and food quality control. However, a high-performance molecular methodology since DNA extraction until detection and quantification of T. cruzi DNA in açai berry pulp is still needed. Herein, a simple DNA extraction methodology was standardized from the supernatant of açai berry pulp stabilized in a 6M Guanidine-HCl/0.2M EDTA buffer. In addition, a multiplex real time qPCR assay, targeting T. cruzi DNA and an Exogenous Internal Positive Control was developed and validated, using reference from all T. cruzi DTUs and commercial samples of açai pulp, from an endemic municipality with previous history of oral Chagas disease outbreak. Thus, a high-sensitivity qPCR assay, that could detect up to 0.01 parasite equivalents/mL in açai, was reached. As of the 45 commercial samples analyzed, 9 (20%) were positive for T. cruzi. This high-sensitive, fast, and easy-to-use molecular assay is compatible with most of the laboratories involved in the investigations of oral Chagas disease outbreaks, representing an important tool to the epidemiology, control, and surveillance of Chagas disease.
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