ArticlePloS one2021
Testing the effect of pollen exine rupture on metabarcoding with Illumina sequencing.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Assessing Botanical Authenticity of Honey Through DNA-Metabarcoding-Based Pollen Analysis: A Preliminary Study.Foods (Basel, Switzerland) · 2026Article
- Method comparison of microscopy, metabarcoding, and multispectral imaging flow cytometry for identification and relative abundance analysis of insect-dispersed pollen.Scientific reports · 2026Article
- Effect of formic acid treatment on Apis mellifera foraging behavior using nanopore metabarcoding technologies.PloS one · 2026Article
- Uncovering the Floral Origins of Honey Bee Pollen in Colombian Tropical Dry Forest: A Low-Cost DNA Barcoding Approach RevealsPlants (Basel, Switzerland) · 2025Article
- Mock Plant Communities and a Large Mammal Case Study Reveal ITS2 Primer Bias Against Graminoids.Ecology and evolution · 2025Article
- Authentication of the Botanical Origin of Honey:Journal of agricultural and food chemistry · 2025Article
- Article
- Conservation and diversity of the pollen microbiome of Pan-American maize using PacBio and MiSeq.Frontiers in microbiology · 2023Article
- Assessing pollen nutrient content: a unifying approach for the study of bee nutritional ecology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Pollen Monitoring by Optical Microscopy and DNA Metabarcoding: Comparative Study and New Insights.International journal of environmental research and public health · 2022Article
- Preservation methods of honey bee-collected pollen are not a source of bias in ITS2 metabarcoding.Environmental monitoring and assessment · 2021Article
- Plant biodiversity assessment through pollen DNA metabarcoding in Natura 2000 habitats (Italian Alps).Scientific reports · 2021Article
- The impact of extraction method and pollen concentration on community composition for pollen metabarcoding.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen metabarcoding has received much attention recently for its potential to increase taxonomic resolution of the identifications of pollen grains necessary for various public health, ecological and environmental inquiry. However, methodologies implemented are widely varied across studies confounding comparisons and casting uncertainty on the reliability of results. In this study, we investigated part of the methodology, the effects of level of exine rupture and lysis incubation time, on the performance of DNA extraction and Illumina sequencing. We examined 15 species of plants from 12 families with pollen that varies in size, shape, and aperture number to evaluate effort necessary for exine rupture. Then created mock communities of 14 of the species from DNA extractions at 4 levels of exine rupture (0, 33, 67, and 100%) and two levels of increased lysis incubation time without exine rupture (2 or 24 hours). Quantities of these DNA extractions displayed a positive correlation between increased rupture and DNA yield, however increasing time of lysis incubation was associated with decreased DNA yield. Illumina sequencing was performed with these artificial community treatments with three common plant DNA barcode regions (rbcL, ITS1, ITS2) with two different primer pairings for ITS2 and rbcL. We found decreased performance in treatments with 0% or 100% exine rupture compared to 33% and 67% rupture, based on deviation from expected proportions and species retrieval, and increased lysis incubation was found to be detrimental to results.
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