ArticleApplied biochemistry and biotechnology2021
Enhanced Lutein Production in Chlamydomonas reinhardtii by Overexpression of the Lycopene Epsilon Cyclase Gene.
Article in Applied biochemistry and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 37 citations in OpenAlex.
- Singlet Oxygen in Plants: Mechanistic Insights into Production, Oxidative Damage, and Stress-Response Signaling.Antioxidants (Basel, Switzerland) · 2026Review
- DcLcyB1 and DcLcyB2, two lycopene β-cyclases with partial functional overlap modulate carotene profiles in carrot roots via distinct catalytic properties.Molecular horticulture · 2026Article
- Genetic Engineering and Innovative Cultivation Strategies for Enhancing the Lutein Production in Microalgae.Marine drugs · 2024Review
- CRISPR/Cas9-Mediated Knockout of the Lycopene ε-Cyclase for Efficient Astaxanthin Production in the Green MicroalgaPlants (Basel, Switzerland) · 2024Article
- Microbial chassis as the platform for production of dihydroxy xanthophyll-based carotenoids: an overview of recent advances in biomanufacturing.World journal of microbiology & biotechnology · 2024Review
- Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase.Biotechnology for biofuels and bioproducts · 2023Article
- A Low-Cost Fertilizer Medium Supplemented with Urea for the Lutein Production ofBioengineering (Basel, Switzerland) · 2023Article
- Article
- Over-expression of a cyanobacterial gene for 1-deoxy-d-xylulose-5-phosphate synthase in the chloroplast ofJournal of King Saud University. Science · 2022Article
- Carotenoid metabolism: New insights and synthetic approaches.Frontiers in plant science · 2022Review
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Authors and funding
10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Chlamydomonas reinhardtii is a well-established microalgal model species with a shorter doubling time, which is a promising natural source for the efficient production of high-value carotenoids. In the microalgal carotenoid biosynthetic pathway, lycopene is converted either into β-carotene by lycopene β-cyclase or into α-carotene by lycopene ε-cyclase (LCYE) and lycopene β-cyclase. In this study, we overexpressed the LCYE gene in C. reinhardtii to estimate its effect on lycopene metabolism and lutein production. Chlamydomonas transformants (CrLCYE#L1, #L5, and #L6) produced significantly increased amounts of lutein per culture (up to 2.6-fold) without a decrease in cell yields. Likewise, the expression levels of LCYE gene in transformants showed a significant increase compared with that of the wild-type strain. These results suggest that LCYE overexpression enhances the conversion of lycopene to α-carotene, which in turn improves lutein productivity. Interestingly, their β-carotene productivity appeared to increase slightly rather than decrease. Considering that the inhibition of the lycopene cyclization steps often induces higher expression in genes upstream of metabolic branches, this result implies that the redirection from β-carotene to α-carotene by LCYE overexpression might also enhance upstream gene expression, thereby leading to auxiliary β-carotene production.
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