Evidence map›Paper›PMID 33527324›Full record

ArticleStem cell reviews and reports2021

RXR Negatively Regulates Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem and Progenitor Cells.

Yuting Jin, Jie Huang, Qin Wang, Jiefeng He, Yincheng Teng, Rongzhen Jiang, Hal E Broxmeyer, Bin Guo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reviews and reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Yuting Jin *Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, 280, Chong-Qing South Road, West Bldg #2, Shanghai, 200025, China.
Jie Huang *Children's Hospital, Fudan University, Minhang, Shanghai, China.
Qin WangDepartment of Gynecology and Obstetrics, The First People's Hospital of Kunshan, Kunshan, 215300, China.
Jiefeng HeDepartment of General Surgery, Shanxi Bethune Hospital, Shanxi Medical University, Taiyuan, Shanxi, 030032, China.
Yincheng TengObstetric Intensive Care Center, The Institute of Obstetrics and Gynecology, Department of Obstetrics and Gynecology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, 200233, Shanghai, China.
Rongzhen JiangObstetric Intensive Care Center, The Institute of Obstetrics and Gynecology, Department of Obstetrics and Gynecology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, 200233, Shanghai, China. jianrzh@sjtu.edu.cn.
Hal E BroxmeyerDepartment of Microbiology and Immunology, Indiana University School of Medicine, 950 West Walnut Street, R2-302, Indianapolis, IN, 46202-5181, USA. hbroxmey@iupui.edu.ORCID http://orcid.org/0000-0001-6575-0476
Bin GuoDepartment of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, 280, Chong-Qing South Road, West Bldg #2, Shanghai, 200025, China. jybinguo@shsmu.edu.cn.
Shanghai Jiao Tong University · CNChildren's Hospital of Fudan University · CNIndiana University School of MedicineJiangsu University · CNShanxi Medical University · CN

Funding

Project-005U54DK106846 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI Reuben Kapur, Karen Elizabeth Pollok · 2015 to 2026
$9.7M
Controlling Influences of Oxygen Tension and CD26/DPP4 Enzymatic Activity on Regulation of Hematopoietic Stem/Progenitor Cells and Hematopoiesis During Health, Ageing, and DiseaseR35HL139599 · NHLBI · INDIANA UNIVERSITY INDIANAPOLIS · PI BROXMEYER, HAL E. · 2018 to 2021
$3.1M
NHLBI NIH HHS R35 HL139599NIDDK NIH HHS U54 DK106846
6 · The paper itself

Abstract

Ex vivo expansion of human cord blood (CB) hematopoietic stem cells (HSCs) is one approach to overcome limited numbers of HSCs in single CB units. However, there is still no worldwide acceptable HSC ex vivo expansion system. A main reason is that we still have very limited knowldege regarding mechanisms underlying maintenance and expansion of CB HSCs. Here we report that retinoid X receptor (RXR) activity is of significance for CB HSC ex vivo expansion. RXR antagonist HX531 significantly promoted ex vivo expansion of CB HSCs and progenitor cells (HPCs). RXR agonist Bexarotene notably suppressed ex vivo expansion of CB HSCs. Activation of RXR by Bexarotene significantly blocked expansion of phenotypic HSCs and HPCs and expressed increased functional HPCs as assessed by colony formation induced by UM171 and SR1. In vivo transplantation experiments in immune-deficient mice demonstrated that HX531 expanded CB HSCs possess long-term reconstituting capacities, and Bexarotene treatment inhibited expansion of functional CB HSCs. RNA-seq analysis revealed that RXR regulates expression of FBP1 (a negative regulator of glucose metabolism) and many genes involved in differentation. ECAR analysis showed that HX531 significantly promoted glycolytic activity of CB CD34

Indexed as

Fetal BloodRetinoid X ReceptorsAnimalsBenzoatesBexaroteneBiphenyl CompoundsHematopoietic Stem CellsHumansMiceBenzoatesBexaroteneBiphenyl Compoundsdiazepinylbenzoic acidRetinoid X ReceptorsCord bloodEx vivo expansionHematopoietic stem and progenitor cellsRetinoid X receptor

Identifiers

PMID33527324
PMCPMC8324505
OpenAlexW3128152992

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.