ArticleApplied microbiology and biotechnology2021
Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR.
Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
- Lipidomic analysis of A1, A2, and heterozygous beta-casein genotypes in bovine milk using gas chromatography-mass spectrometry.Biochemistry and biophysics reports · 2026Article
- Techniques to Detect and Quantify the Bacterial Metalloprotease AprX in Bovine Milk: A Review.Comprehensive reviews in food science and food safety · 2026Review
- Article
- Development of Multiplex qPCR Method for Accurate Detection of Enzyme-Producing Psychrotrophic Bacteria.Foods (Basel, Switzerland) · 2025Article
- An Evaluation of the Sensitivity and Applicability of a Droplet Digital Polymerase Chain Reaction Assay to Simultaneously DetectFoods (Basel, Switzerland) · 2024Article
- Naked-Eye Detection of Food-Borne Pathogens Using Multiplex Hyperbranched Rolling Circle Amplification and Magnetic Particles.Biosensors · 2022Article
- Full-Length SSU rRNA Gene Sequencing Allows Species-Level Detection of Bacteria, Archaea, and Yeasts Present in Milk.Microorganisms · 2021Article
- A Milk Foodomics Investigation into the Effect ofFoods (Basel, Switzerland) · 2021Article
- Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection ofFrontiers in microbiology · 2021Article
- Maternal IgA2 Recognizes Similar Fractions of Colostrum and Fecal Neonatal Microbiota.Frontiers in immunology · 2021Observational
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in raw milk, we developed two triplex qPCR assays taking into account species-dependent differences in AprX activity. Besides five species-specific hydrolysis probes, targeting the aprX gene, a universal rpoB probe was included in the assay to determine the total Pseudomonas counts. For all six probes, linear regression lines between C
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.