ArticleCommunications biology2021
Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Advancements in DNA methylation technologies and their application in cancer diagnosis.Epigenetics · 2025Review
- The technology landscape for detection of DNA methylation in cancer liquid biopsies.Epigenetics · 2025Review
- Epigenomic heterogeneity as a source of tumour evolution.Nature reviews. Cancer · 2025Review
- Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity.Clinical epigenetics · 2023Review
- GraphCpG: imputation of single-cell methylomes based on locus-aware neighboring subgraphs.Bioinformatics (Oxford, England) · 2023Article
- Single cell genomics as a transformative approach for aquaculture research and innovation.Reviews in aquaculture · 2023Review
- Computational Methods for Single-cell DNA Methylome Analysis.Genomics, proteomics & bioinformatics · 2023Review
- DNA methylation QTL mapping across diverse human tissues provides molecular links between genetic variation and complex traits.Nature genetics · 2023Article
- Technologies and applications of single-cell DNA methylation sequencing.Theranostics · 2023Review
- The emerging field of opportunities for single-cell DNA methylation studies in hematology and beyond.Frontiers in molecular biosciences · 2023Article
- GAGAM v1.2: An Improvement on Peak Labeling and Genomic Annotated Gene Activity Matrix Construction.Genes · 2022Article
- Single-Cell DNA Methylation Analysis in Cancer.Cancers · 2022Review
- Epigenetics in Pancreatic Ductal Adenocarcinoma: Impact on Biology and Utilization in Diagnostics and Treatment.Cancers · 2022Review
- Untangling the web of intratumour heterogeneity.Nature cell biology · 2022Review
- Experimental development of the epigenomic library construction method to elucidate the epigenetic diversity and causal relationship between epigenome and transcriptome at a single-cell level.Genomics & informatics · 2022Article
- Pathways of DNA Demethylation.Advances in experimental medicine and biology · 2022Article
- Introduction to Single-Cell DNA Methylation Profiling Methods.Biomolecules · 2021Review
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.
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