ArticleNature cell biology2021
Optogenetic manipulation of cellular communication using engineered myosin motors.
Article in Nature cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 36 citations in OpenAlex.
- Regulation and function of specialized membrane protrusions in intercellular communication.Nature reviews. Molecular cell biology · 2026Review
- Emerging Roles of Cytoneme-Mediated Signaling in Cancer.International journal of molecular sciences · 2026Review
- Article
- Making cells inter-connected for signaling communication: a developmental view of cytonemes.Cell communication and signaling : CCS · 2025Review
- A subcellular map of translational machinery composition and regulation at the single-molecule level.Science (New York, N.Y.) · 2025Article
- The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour.Nature communications · 2025Article
- Diffusion barriers imposed by tissue topology shape Hedgehog morphogen gradients.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- Article
- Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition.Developmental biology · 2024Article
- Efficient generation of functional neurons from mouse embryonic stem cells via neurogenin-2 expression.Nature protocols · 2023Review
- Quantitative insights in tissue growth and morphogenesis with optogenetics.Physical biology · 2023Review
- Cellular and molecular mechanisms of Hedgehog signalling.Nature reviews. Molecular cell biology · 2023Review
- Myosin XV is a negative regulator of signaling filopodia during long-range lateral inhibition.bioRxiv : the preprint server for biology · 2023Article
- Adult tissue-specific stem cell interaction: novel technologies and research advances.Frontiers in cell and developmental biology · 2023Review
- Now that We Got There, What Next?Methods in molecular biology (Clifton, N.J.) · 2023Article
- Precise modulation of embryonic development through optogenetics.Genesis (New York, N.Y. : 2000) · 2022Review
- Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues.Nature communications · 2022Article
- Rebuilding limbs, one cell at a time.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- The use of transgenics in the laboratory axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Review
Corrections and comments
- Commented on by
- Erratum issued
- Erratum issued
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Cells achieve highly efficient and accurate communication through cellular projections such as neurites and filopodia, yet there is a lack of genetically encoded tools that can selectively manipulate their composition and dynamics. Here, we present a versatile optogenetic toolbox of artificial multi-headed myosin motors that can move bidirectionally within long cellular extensions and allow for the selective transport of GFP-tagged cargo with light. Utilizing these engineered motors, we could transport bulky transmembrane receptors and organelles as well as actin remodellers to control the dynamics of both filopodia and neurites. Using an optimized in vivo imaging scheme, we further demonstrate that, upon limb amputation in axolotls, a complex array of filopodial extensions is formed. We selectively modulated these filopodial extensions and showed that they re-establish a Sonic Hedgehog signalling gradient during regeneration. Considering the ubiquitous existence of actin-based extensions, this toolbox shows the potential to manipulate cellular communication with unprecedented accuracy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.