Evidence map›Paper›PMID 33526452›Full record

ArticleMolecular and cellular biology2021

Vitamin K-Dependent γ-Glutamyl Carboxylase in Sertoli Cells Is Essential for Male Fertility in Mice.

Sachiko Shiba, Kazuhiro Ikeda, Kuniko Horie-Inoue, Kotaro Azuma, Tomoka Hasegawa, Norio Amizuka, Tomoaki Tanaka, Toshihiko Takeiwa, Yasuaki Shibata, Takehiko Koji and 1 more

Registry-linked trialOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07330869 (Effects of Warfarin Therapy on Semen Quality, DNA Integrity, Hormonal Profile and Molecular Alterations in Male Patients Undergoing Cardiac Surgery), which is not on this map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07330869 not yet recruitingnot on this mapstarted 2026, after this paper: background citation

Effects of Warfarin Therapy on Semen Quality, DNA Integrity, Hormonal Profile and Molecular Alterations in Male Patients Undergoing Cardiac Surgery: A Prospective Comparative Pilot Study

TypeobservationalSponsorCittà di Lecce Hospital, GVM Care & ResearchRan2026 to 2028Enrolled90ConditionsMale Infertility, Heart Valve DiseaseArmsSemen analysis, sperm DNA fragmentation and hormonal evaluation
3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. TheCurrent issues in molecular biology · 2024
    Article
  5. Gut microbiota supports male reproductionFrontiers in microbiology · 2022
    Review
  6. Testicular aging, male fertility and beyond.Frontiers in endocrinology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Sachiko ShibaDivision of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
Kazuhiro IkedaDivision of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
Kuniko Horie-InoueDivision of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
Kotaro AzumaDepartment of Systems Aging Science and Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
Tomoka HasegawaDepartment of Developmental Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
Norio AmizukaDepartment of Developmental Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
Tomoaki TanakaDepartment of Clinical Cell Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan.
Toshihiko TakeiwaDivision of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
Yasuaki ShibataDepartment of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Takehiko KojiDepartment of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Satoshi InoueDivision of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan sinoue@tmig.or.jp.ORCID 0000-0003-1247-3844
Saitama Medical University · JPHokkaido University · JPNagasaki University · JPTokyo Metropolitan Institute of Gerontology · JPChiba University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

γ-Glutamyl carboxylase (GGCX) is a vitamin K (VK)-dependent enzyme that catalyzes the γ-carboxylation of glutamic acid residues in VK-dependent proteins. The anticoagulant warfarin is known to reduce GGCX activity by inhibiting the VK cycle and was recently shown to disrupt spermatogenesis. To explore GGCX function in the testis, here, we generated Sertoli cell-specific

Indexed as

AnimalsCarbon-Carbon LigasesConnexin 43Germ CellsInfertility, MaleMaleMiceSertoli CellsSpermatogenesisVitamin KCarbon-Carbon LigasesConnexin 43glutamyl carboxylaseVitamin KinfertilityknockoutSertoli celltestisγ-glutamyl carboxylase

Identifiers

PMID33526452
PMCPMC8088131
OpenAlexW3128591108

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.