ArticleInflammation and regeneration2021
Human keratinocyte-derived extracellular vesicles activate the MAPKinase pathway and promote cell migration and proliferation in vitro.
Article in Inflammation and regeneration, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Biomaterial-based extracellular vesicle delivery systems for wound healing: From fabrication to applications.Bioactive materials · 2026Review
- Treatment of eruptive keratoacanthoma in the setting of immunosuppression and atopic dermatitis.JAAD case reports · 2026Article
- Membrane lipid order predicts potency decline in therapeutic extracellular vesicles following handling, storage, and reconstitution stress.Frontiers in bioengineering and biotechnology · 2026Article
- Dehydrated silk fibroin matrices as versatile delivery systems for extracellular vesicles.Frontiers in bioengineering and biotechnology · 2026Article
- Exosomes as Therapeutic and Diagnostic Tools: Advances, Challenges, and Future Directions.Cell biochemistry and biophysics · 2025Review
- D-Tryptophan Promotes Skin Wound Healing via Extracellular Matrix Remodeling in Normal and Diabetic Models.International journal of molecular sciences · 2025Article
- GelMA hydrogel-loaded extracellular vesicles derived from keratinocytes promote skin microvasculature regeneration and wound healing in diabetic mice through activation of the PDGF-induced PI3K/AKT pathway.Cell biology and toxicology · 2025Article
- Quantifying extracellular vesicle heterogeneity: the effect of process conditions on protein cargo for skin therapy.Stem cell research & therapy · 2025Review
- Nanomanaging Chronic Wounds with Targeted Exosome Therapeutics.Pharmaceutics · 2025Review
- Synergistic Effects of Hydrogen Peroxide Preconditioning and Valproic Acid on Hepatic Differentiation of Mesenchymal Stem Cells.Current protein & peptide science · 2025Article
- Exosomes in nanomedicine: a promising cell-free therapeutic intervention in burn wounds.Stem cell research & therapy · 2024Review
- Review
- Innovative Cosmeceutical Ingredients: Harnessing Selenosugar-Linked Hydroxycinnamic Acids for Antioxidant and Wound-Healing Properties.Antioxidants (Basel, Switzerland) · 2024Article
- Cell Type-Specific Extracellular Vesicles and Their Impact on Health and Disease.International journal of molecular sciences · 2024Review
- Therapeutic Applications of Plant-Derived Extracellular Vesicles as Antioxidants for Oxidative Stress-Related Diseases.Antioxidants (Basel, Switzerland) · 2023Review
- Glycolipid Biosurfactants in Skincare Applications: Challenges and Recommendations for Future Exploitation.Molecules (Basel, Switzerland) · 2023Review
- Article
- Effect of Chitosan-Diosgenin Combination on Wound Healing.International journal of molecular sciences · 2023Article
- The Effect ofCurrent issues in molecular biology · 2022Article
- Native and engineered extracellular vesicles for wound healing.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWound healing is a complex biological process and complete skin regeneration is still a critical challenge. Extracellular vesicles (EVs) play essential roles in cell communication and cell regeneration, and recent studies have suggested that EVs may contribute to wound healing, though the molecular mechanisms behind this contribution remain unclear. For these reasons, we decided to use EVs isolated from human keratinocytes (HaCaT) in vitro to determine the potential mechanism of action of EV-derived wound healing.
methodScratch assays were used to determine cell migration and proliferation. Scratched cells were exposed to EVs in multiple conditions to determine how they affect wound healing. Statistical analysis between groups was carried out to using Student's two-sided t test. A p value of < 0.05 was considered statistically significant.
resultWe found that proteomic analysis of purified EVs shows enrichment of proteins associated with cell communication and signal transduction, such as MAPK pathways, and keratinocyte and fibroblast cultures exposed to EVs had higher levels of proliferation, migration, and ERK1/2 and P38 activation. Moreover, we found that treatment with specific ERK1/2 and P38 signaling inhibitors PD98059 and SB239063 impaired EV-mediated cell migration, which suggests that ERK1/2 and P38 signaling is essential for EV-induced wound healing.
conclusionHaCaT cell-derived EVs accelerate the migration and proliferation of human keratinocytes and fibroblasts and may promote wound healing via the activation of MAPKinase pathways. These findings may be key in developing new methods to treat wounds and accelerate wound healing in the future.
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