ArticleMolecular and cellular biology1988
The rat alpha-tropomyosin gene generates a minimum of six different mRNAs coding for striated, smooth, and nonmuscle isoforms by alternative splicing.
Article in Molecular and cellular biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 165 citations in OpenAlex.
- Actin-tropomyosin distribution in non-muscle cells.Journal of muscle research and cell motility · 2020Review
- The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators.Nucleic acids research · 2016Article
- Comprehensive analysis of tropomyosin isoforms in skeletal muscles by top-down proteomics.Journal of muscle research and cell motility · 2016Article
- Top-down mass spectrometry of cardiac myofilament proteins in health and disease.Proteomics. Clinical applications · 2014Review
- In-depth proteomic analysis of human tropomyosin by top-down mass spectrometry.Journal of muscle research and cell motility · 2013Article
- MBNL1 and PTB cooperate to repress splicing of Tpm1 exon 3.Nucleic acids research · 2013Article
- Top-down targeted proteomics for deep sequencing of tropomyosin isoforms.Journal of proteome research · 2013Article
- PEP1 of Arabis alpina is encoded by two overlapping genes that contribute to natural genetic variation in perennial flowering.PLoS genetics · 2012Article
- Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.American journal of physiology. Cell physiology · 2011Article
- Internal and external paralogy in the evolution of tropomyosin genes in metazoans.Molecular biology and evolution · 2010Article
- Molecular and functional characterization of a novel cardiac-specific human tropomyosin isoform.Circulation · 2010Article
- Requirement of TCTG(G/C) Direct Repeats and Overlapping GATA Site for Maintaining the Cardiac-Specific Expression of Cardiac troponin T in Developing and Adult Mice.Anatomical record (Hoboken, N.J. : 2007) · 2008Article
- Activation of alpha-tropomyosin exon 2 is regulated by the SR protein 9G8 and heterogeneous nuclear ribonucleoproteins H and F.Molecular and cellular biology · 2006Article
- An apparent pseudo-exon acts both as an alternative exon that leads to nonsense-mediated decay and as a zero-length exon.Molecular and cellular biology · 2006Article
- Competition of PTB with TIA proteins for binding to a U-rich cis-element determines tissue-specific splicing of the myosin phosphatase targeting subunit 1.RNA (New York, N.Y.) · 2005Article
- The PTB interacting protein raver1 regulates alpha-tropomyosin alternative splicing.The EMBO journal · 2003Article
- A splicing silencer that regulates smooth muscle specific alternative splicing is active in multiple cell types.Nucleic acids research · 2002Article
- Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibers by clonal expansion.The Journal of cell biology · 2000Article
- Four isoforms of serum response factor that increase or inhibit smooth-muscle-specific promoter activity.The Biochemical journal · 2000Article
- The PDZ domain of the LIM protein enigma binds to beta-tropomyosin.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tropomyosin (TM), a ubiquitous protein, is a component of the contractile apparatus of all cells. In nonmuscle cells, it is found in stress fibers, while in sarcomeric and nonsarcomeric muscle, it is a component of the thin filament. Several different TM isoforms specific for nonmuscle cells and different types of muscle cell have been described. As for other contractile proteins, it was assumed that smooth, striated, and nonmuscle isoforms were each encoded by different sets of genes. Through the use of S1 nuclease mapping, RNA blots, and 5' extension analyses, we showed that the rat alpha-TM gene, whose expression was until now considered to be restricted to muscle cells, generates many different tissue-specific isoforms. The promoter of the gene appears to be very similar to other housekeeping promoters in both its pattern of utilization, being active in most cell types, and its lack of any canonical sequence elements. The rat alpha-TM gene is split into at least 13 exons, 7 of which are alternatively spliced in a tissue-specific manner. This gene arrangement, which also includes two different 3' ends, generates a minimum of six different mRNAs each with the capacity to code for a different protein. These distinct TM isoforms are expressed specifically in nonmuscle and smooth and striated (cardiac and skeletal) muscle cells. The tissue-specific expression and developmental regulation of these isoforms is, therefore, produced by alternative mRNA processing. Moreover, structural and sequence comparisons among TM genes from different phyla suggest that alternative splicing is evolutionarily a very old event that played an important role in gene evolution and might have appeared concomitantly with or even before constitutive splicing.
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