Evidence map›Paper›PMID 33525574›Full record

ArticleBiomolecules2021

BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms.

Zorana Lopandić, Luka Dragačević, Dragan Popović, Uros Andjelković, Rajna Minić, Marija Gavrović-Jankulović

Open access · goldAbstract read
In one paragraph

Article in Biomolecules, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Development of molecular sensors based on fluorescent proteins for polarized macrophages identification.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024
    Article
  3. Review
  4. Lectins and lectibodies: potential promising antiviral agents.Cellular & molecular biology letters · 2022
    Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Zorana LopandićDepartment of Biochemistry, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia.ORCID 0000-0002-8034-4086
Luka DragačevićInstitute of Virology, Vaccines and Sera, 11152 Belgrade, Serbia.
Dragan PopovićInstitute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.ORCID 0000-0001-6776-7271
Uros AndjelkovićInstitute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia.
Rajna MinićInstitute of Virology, Vaccines and Sera, 11152 Belgrade, Serbia.ORCID 0000-0003-2941-9246
Marija Gavrović-JankulovićDepartment of Biochemistry, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia.ORCID 0000-0002-8591-4391
University of Belgrade · RSInstitute of Virology, Vaccines and Sera “Torlak” · RSUniversity of Rijeka · HR

Funding

Bilateral scientific project financed by Ministries of Sciences of Croatia and Serbia. 8Ministry of Education, Science and Technological Development, Republic of Serbia. Contract number: 451-03-68/2020-14/200168, Contract number: 451-03-68/2020-14/200177, and Contract number: 451-03-68/2020-14/200026
6 · The paper itself

Abstract

Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi. For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in

Indexed as

Escherichia coliGreen Fluorescent ProteinsLectinsMannosePolysaccharidesCrystallography, X-RayFlow CytometryMusaPeptidesPlant LectinsProtein BindingProtein ConformationProtein Structure, SecondaryGreen Fluorescent ProteinsLectinsMannosePeptidesPlant LectinsPolysaccharidesbanana lectineGFPflorescence-linked lectin sorbent assayfluorescenceinfluenza vaccineSalmonella strainsviral glycoproteins

Identifiers

PMID33525574
PMCPMC7912117
OpenAlexW3121253403

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.