Evidence map›Paper›PMID 33525381›Full record

ArticleCells2021

Osteoarthritic Subchondral Bone Release Exosomes That Promote Cartilage Degeneration.

Xiaoxin Wu, Ross Crawford, Yin Xiao, Xinzhan Mao, Indira Prasadam

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 61 citations in OpenAlex.

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  12. From pathogenesis to therapy: extracellular vesicles in osteoarthritis.Frontiers in bioengineering and biotechnology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Xiaoxin WuDepartment of Orthopaedic Surgery, the Second Xiangya Hospital, Central South University, Changsha 410011, China.
Ross CrawfordInstitute of Health and Biomedical Innovation, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane 4059, Australia.
Yin XiaoInstitute of Health and Biomedical Innovation, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane 4059, Australia.ORCID 0000-0003-1785-3491
Xinzhan MaoDepartment of Orthopaedic Surgery, the Second Xiangya Hospital, Central South University, Changsha 410011, China.
Indira PrasadamInstitute of Health and Biomedical Innovation, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane 4059, Australia.
Queensland University of Technology · AUCentral South University · CN

Funding

National Natural Science Foundation of China 81371997
6 · The paper itself

Abstract

Altered subchondral bone and articular cartilage interactions have been implicated in the pathogenesis of osteoarthritis (OA); however, the mechanisms remain unknown. Exosomes are membrane-derived vesicles that have recently been recognized as important mediators of intercellular communication. Herein, we investigated if OA subchondral bone derived exosomes alter transcriptional and bioenergetic signatures of chondrocytes. Exosomes were isolated and purified from osteoblasts of nonsclerotic or sclerotic zones of human OA subchondral bone and their role on the articular cartilage chondrocytes was evaluated by measuring the extent of extracellular matrix production, cellular bioenergetics, and the expression of chondrocyte activity associated marker genes. Exosomal microRNAs were analyzed using RNA sequencing and validated by quantitative real-time PCR and loss-of-function. In coculture studies, chondrocytes internalized OA sclerotic subchondral bone osteoblast derived exosomes and triggered catabolic gene expression and reduced chondrocyte-specific marker expression a phenomenon that is often observed in OA cartilage. RNA sequencing and miRNA profiling have identified miR-210-5p, which is highly enriched in OA sclerotic subchondral bone osteoblast exosomes, triggered the catabolic gene expression in articular cartilage chondrocytes. Importantly, we demonstrate that miR-210-5p suppresses the oxygen consumption rate of chondrocytes, altering their bioenergetic state that is often observed in OA conditions. These effects were markedly inhibited by the addition of a miR-210-5p inhibitor. Our study indicates that exosomes released by OA sclerotic subchondral bone osteoblasts plays a critical role in progression of cartilage degeneration and might be a potential target for therapeutic intervention in OA.

Indexed as

AerobiosisAged, 80 and overBone and BonesCartilage, ArticularCell CommunicationCell RespirationChondrocytesExosomesFemaleGene Expression RegulationHumansHypertrophyMaleMicroRNAsOsteoarthritisOsteoblastsMicroRNAsMIRN210 microRNA, humancartilagecell-interactionsexosomesmiRNAosteoarthritissubchondral bone

Identifiers

PMID33525381
PMCPMC7911822
OpenAlexW3126989368

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.