ArticleScience advances2021
Polarization-sensitive stimulated Raman scattering imaging resolves amphotericin B orientation in
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Liposomal antimicrobials in the fight against bacterial and fungal pathogens: Clinical successes and development challenges.International journal of pharmaceutics: X · 2026Review
- Label-Free Visualization of the Antifungal Polyene Drug, Nystatin, in Biological Membranes Using Raman Microscopy.Analytical chemistry · 2026Article
- Review
- First report of Candidozyma auris in Vietnam.BMC microbiology · 2026Article
- Fluorescent Probes Derived from the Polyene Class of Antifungal Drugs Reveal Distinct Localization Patterns and Resistance-Associated Vacuolar Sequestration in Candida Species.Angewandte Chemie (International ed. in English) · 2026Article
- Enhanced Skin Delivery of Amphotericin B: Development and Evaluation of Polymeric Nanoparticles Using a Porcine Ear Model.ACS omega · 2025Article
- Raman Imaging of an Amphotericin B Potentiator in Single-CellThe journal of physical chemistry. B · 2025Article
- Identification of a Fusobacterial RNA-binding protein involved in host small RNA-mediated growth inhibition.International journal of oral science · 2025Article
- Quantum theory of stimulated Raman scattering microscopy.Chemical physics reviews · 2025Review
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Gladiolin produced by pathogenicmBio · 2024Article
- Insight into Virulence and Mechanisms of Amphotericin B Resistance in theJournal of fungi (Basel, Switzerland) · 2024Review
- Catalase Deactivation Increases Dermatophyte Sensitivity to ROS Sources.Journal of fungi (Basel, Switzerland) · 2024Article
- Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging.Analytical chemistry · 2024Review
- How Does the Antibiotic Amphotericin B Enter Membranes and What Does It Do There?The journal of physical chemistry letters · 2024Article
- Anti-Infection of Oral Microorganisms from Herbal Medicine ofDrug design, development and therapy · 2024Review
- Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications.Chemical & biomedical imaging · 2023Review
- Enhanced Antifungal Activity of Amphotericin B Bound to Albumin: A "Trojan Horse" Effect of the Protein.The journal of physical chemistry. B · 2023Article
- Artificial intelligence-aided rapid and accurate identification of clinical fungal infections by single-cell Raman spectroscopy.Frontiers in microbiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Ergosterol-targeting amphotericin B (AmB) is the first line of defense for life-threatening fungal infections. Two models have been proposed to illustrate AmB assembly in the cell membrane; one is the classical ion channel model in which AmB vertically forms transmembrane tunnel and the other is a recently proposed sterol sponge model where AmB is laterally adsorbed onto the membrane surface. To address this controversy, we use polarization-sensitive stimulated Raman scattering from fingerprint C═C stretching vibration to visualize AmB, ergosterol, and lipid in single fungal cells. Intracellular lipid droplet accumulation in response to AmB treatment is found. AmB is located in membrane and intracellular droplets. In the 16 strains studied, AmB residing inside cell membrane was highly ordered, and its orientation is primarily parallel to phospholipid acyl chains, supporting the ion channel model. Label-free imaging of AmB and chemical contents offers an analytical platform for developing low-toxicity, resistance-refractory antifungal agents.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.