ArticleScience advances2021
Full-field fluorescence lifetime dual-comb microscopy using spectral mapping and frequency multiplexing of dual-comb optical beats.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Scan-less microscopy based on acousto-optic encoded illumination.Nanophotonics (Berlin, Germany) · 2024Article
- Dual-comb quartz-enhanced photoacoustic spectroscopy.Photoacoustics · 2022Article
- Dual chirped microcomb based parallel ranging at megapixel-line rates.Nature communications · 2022Article
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces.Frontiers in cell and developmental biology · 2021Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust to photobleaching. However, since most FLIMs are based on point-to-point measurements, mechanical scanning of a focal spot is needed for forming an image, which hampers rapid imaging. Here, we demonstrate scan-less full-field FLIM based on a one-to-one correspondence between two-dimensional (2D) image pixels and frequency-multiplexed radio frequency (RF) signals. A vast number of dual-comb optical beats between dual optical frequency combs are effectively adopted for 2D spectral mapping and high-density frequency multiplexing in the RF region. Bimodal images of fluorescence amplitude and lifetime are obtained with high quantitativeness from amplitude and phase spectra of fluorescence RF comb modes without the need for mechanical scanning. The parallelized FLIM will be useful for rapid quantitative fluorescence imaging in life science.
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