Evidence map›Paper›PMID 33522648›Full record

ArticleCell proliferation2021

Transient characteristics of universal cells on human-induced pluripotent stem cells and their differentiated cells derived from foetal stem cells with mixed donor sources.

Tzu-Cheng Sung, Yi-Peng Jiang, Jhe-Yu Hsu, Qing-Dong Ling, Hao Chen, Suresh S Kumar, Yung Chang, Shih-Tien Hsu, Qingsong Ye, Akon Higuchi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tzu-Cheng SungSchool of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, China.ORCID https://orcid.org/0000-0003-4052-8048
Yi-Peng JiangDepartment of Chemical and Materials Engineering, National Central University, Taoyuan, Taiwan.
Jhe-Yu HsuDepartment of Chemical and Materials Engineering, National Central University, Taoyuan, Taiwan.
Qing-Dong LingCathay Medical Research Institute, Cathay General Hospital, Taipei, Taiwan.
Hao ChenSchool of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
Suresh S KumarDepartment of Biotechnology, Bharath Institute of Higher Education and Research, Chennai, India.
Yung ChangDepartment of Chemical Engineering and R&D Center for Membrane Technology, Chung Yuan Christian University, Taoyuan, Taiwan.
Shih-Tien HsuDepartment of Internal Medicine, Taiwan Landseed Hospital, Pingjen City, Taiwan.
Qingsong YeCenter of Regenerative Medicine, Renmin Hospital of Wuhan University, Hubei, China.ORCID https://orcid.org/0000-0002-2868-9247
Akon HiguchiSchool of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, China.ORCID https://orcid.org/0000-0003-2970-8531

Funding

Landseed Hospital Project NCU-LSH-108-B-003Landseed Hospital Project NCU-LSH-109-B-00Ministry of Education, Culture, Sports, Science and Technology 18K05251Ministry of Science and Technology, Taiwan 108-2221-E-008-037Ministry of Science and Technology, Taiwan 108-2221-E-008-057Ministry of Science and Technology, Taiwan 109-2221-E-008-032
6 · The paper itself

Abstract

introductionIt is important to prepare 'hypoimmunogenic' or 'universal' human pluripotent stem cells (hPSCs) with gene-editing technology by knocking out or in immune-related genes, because only a few hypoimmunogenic or universal hPSC lines would be sufficient to store for their off-the-shelf use. However, these hypoimmunogenic or universal hPSCs prepared previously were all genetically edited, which makes laborious processes to check and evaluate no abnormal gene editing of hPSCs.

methodsUniversal human-induced pluripotent stem cells (hiPSCs) were generated without gene editing, which were reprogrammed from foetal stem cells (human amniotic fluid stem cells) with mixing 2-5 allogenic donors but not with single donor. We evaluated human leucocyte antigen (HLA)-expressing class Ia and class II of our hiPSCs and their differentiated cells into embryoid bodies, cardiomyocytes and mesenchymal stem cells. We further evaluated immunogenic response of transient universal hiPSCs with allogenic mononuclear cells from survival rate and cytokine production, which were generated by the cells due to immunogenic reactions.

resultsOur universal hiPSCs during passages 10-25 did not have immunogenic reaction from allogenic mononuclear cells even after differentiation into cardiomyocytes, embryoid bodies and mesenchymal stem cells. Furthermore, the cells including the differentiated cells did not express HLA class Ia and class II. Cardiomyocytes differentiated from transient universal hiPSCs at passage 21-22 survived and continued beating even after treatment with allogenic mononuclear cells.

Indexed as

Cell DifferentiationEmbryoid BodiesFetal Stem CellsGene EditingHumansInduced Pluripotent Stem CellsMesenchymal Stem CellsMyocytes, CardiacPluripotent Stem Cellshuman-induced pluripotent stem cellsregenerative medicinestem cellsuniversal cells

Identifiers

PMID33522648
PMCPMC7941237

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.