Evidence map›Paper›PMID 33522227›Full record

ArticleACS chemical neuroscience2021

Genetic Probe for Visualizing Glutamatergic Synapses and Vesicles by 3D Electron Microscopy.

Thomas Steinkellner, Matthew Madany, Matthias G Haberl, Vivien Zell, Carolina Li, Junru Hu, Mason Mackey, Ranjan Ramachandra, Stephen Adams, Mark H Ellisman and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS chemical neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 3 countries.

Thomas SteinkellnerDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Matthew MadanyDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Matthias G HaberlDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Vivien ZellDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Carolina LiCenter for Research in Biological Systems, National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, California 92093, United States.
Junru HuDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Mason MackeyDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Ranjan RamachandraDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Stephen AdamsDepartment of Pharmacology, University of California, San Diego, La Jolla California 92093, United States.ORCID 0000-0002-4725-2091
Mark H EllismanDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Thomas S HnaskoDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.
Daniela BoassaDepartment of Neurosciences, University of California, San Diego, La Jolla, California 92093, United States.ORCID 0000-0003-4631-4139
University of California San Diego · US

Funding

IVEM and Image Analysis ResourceP41GM103412 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2012 to 2019
$17.9M
New Probe and Methods for Correlated LM & EMR01GM086197 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ADAMS, STEPHEN ROY, BOASSA, DANIELA · 2008 to 2020
$8.3M
Role of novel VTA neurons in addiction-related behaviorsR01DA036612 · NIDA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Thomas Hnasko · 2014 to 2026
$5.6M
National Center for Microscopy and Imaging Research: A BRAIN Technology Integration and Dissemination ResourceU24NS120055 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2021 to 2025
$5.4M
The National Center for Microscopy and Imaging Research, a Community-wide Scientific ResourceR24GM137200 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2020 to 2022
$3.0M
Developing new tools for high throughput analysis of microcircuits and synapse ultrastructure using tagged vesicular transporters and deep learning.RF1MH120685 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BOASSA, DANIELA, HNASKO, THOMAS · 2019 to 2019
$2.5M
Regulation of VGLUT2 in aging-related neurodegenerative disordersK99AG059834 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI STEINKELLNER, THOMAS · 2018 to 2019
$236k
Austrian Science Fund FWF J 3656NIA NIH HHS K99 AG059834NIDA NIH HHS R01 DA036612NIGMS NIH HHS P41 GM103412NIGMS NIH HHS R01 GM086197NIGMS NIH HHS R24 GM137200NIMH NIH HHS RF1 MH120685NINDS NIH HHS U24 NS120055
6 · The paper itself

Abstract

Communication between neurons relies on the release of diverse neurotransmitters, which represent a key-defining feature of a neuron's chemical and functional identity. Neurotransmitters are packaged into vesicles by specific vesicular transporters. However, tools for labeling and imaging synapses and synaptic vesicles based on their neurochemical identity remain limited. We developed a genetically encoded probe to identify glutamatergic synaptic vesicles at the levels of both light and electron microscopy (EM) by fusing the mini singlet oxygen generator (miniSOG) probe to an intralumenal loop of the vesicular glutamate transporter-2. We then used a 3D imaging method, serial block-face scanning EM, combined with a deep learning approach for automatic segmentation of labeled synaptic vesicles to assess the subcellular distribution of transporter-defined vesicles at nanometer scale. These tools represent a new resource for accessing the subcellular structure and molecular machinery of neurotransmission and for transmitter-defined tracing of neuronal connectivity.

Indexed as

NeuronsSynapsesAnimalsGlutamic AcidMiceMicroscopy, ElectronSynaptic VesiclesVesicular Glutamate Transport Protein 1Vesicular Glutamate Transport Protein 2Glutamic AcidVesicular Glutamate Transport Protein 1Vesicular Glutamate Transport Protein 23D electron microscopy (EM)CDeep3Mdeep learninggenetic EM probeglutamateglutamatergic synapseminiSOGneurotransmissionsynaptic vesiclesventral tegmental area (VTA)vesicular glutamate transporter (VGLUT2)

Identifiers

PMID33522227
PMCPMC7899175
OpenAlexW3127230241

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.