ArticleThe New phytologist2021
A new Cannabis genome assembly associates elevated cannabidiol (CBD) with hemp introgressed into marijuana.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
100 citing papers in PubMed, 174 citations in OpenAlex.
- Towards an Omics-Guided Framework for Microbial Biological Control of Fungal and Oomycete Diseases inInternational journal of molecular sciences · 2026Review
- Four haplotype-resolved genome assemblies and a reference-free 66-haplotype pangenome graph for Cannabis sativa.Scientific data · 2026Article
- Back to the roots: Cannabis sativa L. root metabolism, microbiomes, and biotechnological potential.Applied microbiology and biotechnology · 2026Review
- Genetic Advances inPlants (Basel, Switzerland) · 2026Review
- Transcriptomic dissection of floral identity and trichome-derived metabolite pathways in Cannabis sativa L.Planta · 2026Article
- Transcriptome assemblies for two drug-type cannabis chemotypes by long-read RNA sequencing.The plant genome · 2026Article
- Genetic architecture of phenological, morphological, and phytochemical traits in Cannabis landraces.The plant genome · 2026Article
- Extensive variation between chromosomes of North American and European hop.Nature communications · 2026Article
- An X-linked sex determination mechanism in cannabis and hop.Nature communications · 2026Article
- Cannabidiol and other non-psychotropic cannabinoids from Cannabis sativa as therapeutics for microglial-mediated neuroinflammation and neurodegeneration.Journal of cannabis research · 2026Review
- Advances in Functional Genomics and Biotechnology for Enhancing Therapeutic Potential of Medicinal Plants.International journal of molecular sciences · 2026Review
- The Structure of the Chemotype Determining Locus inPlant direct · 2026Article
- Hierarchical Chemotaxonomic Differentiation in Cannabis Chemovars Using Quantitative HPLC Cannabinoid Profiling and Multivariate Chemometrics.Plants (Basel, Switzerland) · 2026Article
- Resurrected Ancestral Cannabis Enzymes Unveil the Origin and Functional Evolution of Cannabinoid Synthases.Plant biotechnology journal · 2026Article
- Insights into glandular trichome biology from analysis of organ-specific gene expression programmes in cannabis, hop and tomato.The Plant journal : for cell and molecular biology · 2026Article
- Micropropagation of Cannabis sativa: genetic and epigenetic stability assessment over multiple generations.Journal of cannabis research · 2026Article
- A trio-binning approach for genome assembly reveals extensive structural variation between two Cannabis cultivars: Punto Rojo and Cherry Pie.G3 (Bethesda, Md.) · 2026Article
- Sex-specific ethylene responses drive floral sexual plasticity in Cannabis sativa.The Plant journal : for cell and molecular biology · 2026Article
- Population Structure of Genotypes and Genome-Wide Association Studies of Cannabinoids and Terpenes Synthesis in Hemp (Plants (Basel, Switzerland) · 2026Article
- Comparative transcriptome analysis reveals putative transcription factors components of the regulatory network for cannabidiol biosynthesis inFrontiers in plant science · 2026Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Demand for cannabidiol (CBD), the predominant cannabinoid in hemp (Cannabis sativa), has favored cultivars producing unprecedented quantities of CBD. We investigated the ancestry of a new cultivar and cannabinoid synthase genes in relation to cannabinoid inheritance. A nanopore-based assembly anchored to a high-resolution linkage map provided a chromosome-resolved genome for CBDRx, a potent CBD-type cultivar. We measured cannabinoid synthase expression by cDNA sequencing and conducted a population genetic analysis of diverse Cannabis accessions. Quantitative trait locus mapping of cannabinoids in a hemp × marijuana segregating population was also performed. Cannabinoid synthase paralogs are arranged in tandem arrays embedded in long terminal repeat retrotransposons on chromosome 7. Although CBDRx is predominantly of marijuana ancestry, the genome has cannabidiolic acid synthase (CBDAS) introgressed from hemp and lacks a complete sequence for tetrahydrocannabinolic acid synthase (THCAS). Three additional genomes, including one with complete THCAS, confirmed this genomic structure. Only cannabidiolic acid synthase (CBDAS) was expressed in CBD-type Cannabis, while both CBDAS and THCAS were expressed in a cultivar with an intermediate tetrahydrocannabinol (THC) : CBD ratio. Although variation among cannabinoid synthase loci might affect the THC : CBD ratio, variability among cultivars in overall cannabinoid content (potency) was also associated with other chromosomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.