ArticleApplied microbiology and biotechnology2021
A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine.
Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 20 citations in OpenAlex.
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- Cell walls of filamentous fungi - challenges and opportunities for biotechnology.Applied microbiology and biotechnology · 2025Review
- Enhancement of polymyxin B1 production by an artificial microbial consortium ofSynthetic and systems biotechnology · 2024Article
- Engineered microbial consortia for next-generation feedstocks.Biotechnology notes (Amsterdam, Netherlands) · 2024Article
- From Aquaculture to Aquaculture: Production of the Fish Feed Additive Astaxanthin byMolecules (Basel, Switzerland) · 2023Article
- Enhancing plant growth promoting rhizobacterial activities through consortium exposure: A review.Frontiers in bioengineering and biotechnology · 2023Review
- Metabolic Engineering ofMicroorganisms · 2022Article
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds byFrontiers in microbiology · 2022Review
- Customized chitooligosaccharide production-controlling their lengthFrontiers in bioengineering and biotechnology · 2022Article
- Co-cultivation, Co-culture, Mixed Culture, and Microbial Consortium of Fungi: An Understudied Strategy for Biomass Conversion.Frontiers in microbiology · 2021Article
- Utilization of a Wheat Sidestream for 5-Aminovalerate Production inFrontiers in bioengineering and biotechnology · 2021Article
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin is an abundant waste product from shrimp and mushroom industries and as such, an appropriate secondary feedstock for biotechnological processes. However, chitin is a crystalline substrate embedded in complex biological matrices, and, therefore, difficult to utilize, requiring an equally complex chitinolytic machinery. Following a bottom-up approach, we here describe the step-wise development of a mutualistic, non-competitive consortium in which a lysine-auxotrophic Escherichia coli substrate converter cleaves the chitin monomer N-acetylglucosamine (GlcNAc) into glucosamine (GlcN) and acetate, but uses only acetate while leaving GlcN for growth of the lysine-secreting Corynebacterium glutamicum producer strain. We first engineered the substrate converter strain for growth on acetate but not GlcN, and the producer strain for growth on GlcN but not acetate. Growth of the two strains in co-culture in the presence of a mixture of GlcN and acetate was stabilized through lysine cross-feeding. Addition of recombinant chitinase to cleave chitin into GlcNAc
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